Validation Data Gallery

Filter: AllWBIHCIF-PIF-FroFC (Intra)

WB analysis of HeLa cells using 55259-1-AP
WB result of VDAC1 antibody (55259-1-AP, 1:2000) with si-Control and si-VDAC1 transfected HeLa cells.
WB analysis using 55259-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis using 55259-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.
WB analysis using 55259-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse liver using 55259-1-AP
mouse liver tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis using 55259-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 55259-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of human skeletal muscle using 55259-1-AP
human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 55259-1-AP
A431 cells were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of mouse heart using 55259-1-AP
mouse heart tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
WB analysis of rat liver using 55259-1-AP
rat liver tissue were subjected to SDS PAGE followed by western blot with 55259-1-AP (VDAC1/Porin antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.
IHC staining of human heart using 55259-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human heart using 55259-1-AP
Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver using 55259-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human liver using 55259-1-AP
Immunohistochemical analysis of paraffin-embedded human liver using 55259-1-AP (VDAC1/Porin antibody) at dilution of 1:50 (under 40x lens).
IF Staining of human liver cancer using 55259-1-AP
Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using VDAC1/Porin antibody (55259-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
IF Staining of mouse liver using 55259-1-AP
Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse liver tissue using VDAC1/Porin antibody (55259-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).
FC experiment of HepG2 using 55259-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human VDAC1/Porin (55259-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Featured Product
KD/KO Validated

VDAC1/Porin Polyclonal antibody

VDAC1/Porin Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Cat No. 55259-1-AP

Publications(177)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA, PLA

Synonyms

Porin, VDAC1, hVDAC1, Plasmalemmal porin, PORIN 31 HL

Formulation: PBS and Azide

PBS and Azide

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647 CoraLite®555

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (177)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	RAW 264.7 cells, rat liver tissue, HepG2 cells, human skeletal muscle tissue, HEK-293 cells, HeLa cells, mouse liver tissue, A431 cells, mouse heart tissue, ROS1728 cells, mouse brain tissue, rat brain tissue, rat heart tissue, NIH/3T3 cells
Positive IHC detected in	human heart tissue, human liver tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human liver cancer tissue
Positive IF-Fro detected in	mouse liver tissue
Positive FC (Intra) detected in	HepG2 cells

This antibody is not recommended for immunocytofluorescent assays.

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:6000
Immunohistochemistry (IHC)	IHC : 1:50-1:500
Immunofluorescence (IF)-P	IF-P : 1:50-1:500
Immunofluorescence (IF)-FRO	IF-FRO : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 1 publications below
WB	See 161 publications below
IHC	See 6 publications below
IF	See 13 publications below
IP	See 3 publications below
FC	See 1 publications below
CoIP	See 4 publications below

Product Information

55259-1-AP targets VDAC1/Porin in WB, IHC, IF-P, IF-Fro, FC (Intra), CoIP, ELISA, PLA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, canine, monkey, chicken, bovine, hamster, duck
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide 相同性解析による交差性が予測される生物種
Full Name	voltage-dependent anion channel 1
Calculated molecular weight	31 kDa
Observed molecular weight	31 kDa
GenBank accession number	NM_003374
Gene Symbol	Porin
Gene ID (NCBI)	7416
RRID	AB_10837225
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P21796
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.

Protocols

Product Specific Protocols
WB protocol for VDAC1/Porin antibody 55259-1-AP	Download protocol
IHC protocol for VDAC1/Porin antibody 55259-1-AP	Download protocol
IF protocol for VDAC1/Porin antibody 55259-1-AP	Download protocol
FC protocol for VDAC1/Porin antibody 55259-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion Authors - Lina Herhaus View Article
human	WB	Nat Commun MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration Authors - Jianxiong Chen View Article
human	WB	Mol Cell Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology Authors - Gayathri Muthukumar View Article
monkey	WB	Nat Commun An ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D-TSG101 interactions. Authors - Jingru Wang View Article
human	WB	Nucleic Acids Res The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases. Authors - Gui-Xin Peng View Article
	IHC	Mol Biol Evol Functional Attenuation of UCP1 as the Potential Mechanism for Thickened Blubber Layer in Cetaceans Authors - Ming Zhou View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis of HeLa cells using 55259-1-AP

WB analysis using 55259-1-AP

WB analysis using 55259-1-AP

WB analysis using 55259-1-AP

WB analysis of mouse liver using 55259-1-AP

WB analysis using 55259-1-AP

WB analysis of HepG2 using 55259-1-AP

WB analysis of human skeletal muscle using 55259-1-AP

WB analysis of A431 using 55259-1-AP

WB analysis of mouse heart using 55259-1-AP

WB analysis of rat liver using 55259-1-AP

IHC staining of human heart using 55259-1-AP

IHC staining of human heart using 55259-1-AP

IHC staining of human liver using 55259-1-AP

IHC staining of human liver using 55259-1-AP

IF Staining of human liver cancer using 55259-1-AP

IF Staining of mouse liver using 55259-1-AP

FC experiment of HepG2 using 55259-1-AP

VDAC1/Porin Polyclonal antibody

VDAC1/Porin Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

-/ -

Tested Applications

This antibody is not recommended for immunocytofluorescent assays.

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

IRGQ-mediated autophagy in MHC class I quality control promotes tumor immune evasion

MYG1 drives glycolysis and colorectal cancer development through nuclear-mitochondrial collaboration

Triaging of α-helical proteins to the mitochondrial outer membrane by distinct chaperone machinery based on substrate topology

An ESCRT-dependent step in fatty acid transfer from lipid droplets to mitochondria through VPS13D-TSG101 interactions.

The human tRNA taurine modification enzyme GTPBP3 is an active GTPase linked to mitochondrial diseases.

Functional Attenuation of UCP1 as the Potential Mechanism for Thickened Blubber Layer in Cetaceans