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  • Featured Product
  • KD/KO Validated

CoraLite®555-conjugated VDAC1/Porin Polyclonal antibody

VDAC1/Porin Polyclonal Antibody for IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Conjugate

CoraLite®555 Fluorescent Dye

Cat no : CL555-55259

Synonyms

Porin, VDAC1, Porin 31HM, Porin 31HL, PORIN 31 HL

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human liver cancer tissue using CoraLite®555-conjugated VDAC1/Porin Polyclonal ant (CL555-55259)

    IF Staining of human liver cancer using CL555-55259

    Immunofluorescent analysis of (4% PFA) fixed human liver cancer tissue using CoraLite®555 VDAC1/Porin antibody (CL555-55259) at dilution of 1:200.

  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite®555-conjugated VDAC1/Porin Polyclonal ant (CL555-55259)

    FC experiment of HepG2 using CL555-55259

    1x10^6 HepG2 cells were intracellularly stained with 0.8 ug CoraLite®555-conjugated VDAC1/Porin Polyclonal antibody (CL555-55259) (red), or 0.8 ug CoraLite®555-conjugated Rabbit IgG control Rabbit PolyAb (CL555-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF-P detected inhuman liver cancer tissue
Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-55259 targets VDAC1/Porin in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name voltage-dependent anion channel 1
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberNM_003374
Gene symbol Porin
Gene ID (NCBI) 7416
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VDAC1, also named as VDAC, porin 31HM, porin 31HL and plasmalemmal porin, belongs to the eukaryotic mitochondrial porin family. It adopts an open conformation at low or zero membrane potential and a closed conformation at potentials above 30-40 mV, to form a channel through the mitochondrial outer membrane and also the plasma membrane. Unlike other membrane transport proteins, porins are large enough to allow passive diffusion. Studies have shown that VDAC1 is subject to both phosphorylation and acetylation (PMID: 23233904). The apparent molecular weight of VDAC1 is 30-37 kDa (PMID: 14573604; 23754752; 25681439). Hypoxic conditions were found to trigger cleavage of the VDAC1 C-terminal to yield a 26-kDa truncated but active form (PMID: 22389449; 23233904). This antibody is specific to VDAC1.

Protocols

Product Specific Protocols
IF protocol for CL555 VDAC1/Porin antibody CL555-55259Download protocol
FC protocol for CL555 VDAC1/Porin antibody CL555-55259Download protocol
Standard Protocols
Click here to view our Standard Protocols