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Species Reactivity

Species Reactivity

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  • Featured Product
  • KD/KO Validated

VDAC3 Recombinant antibody, PBS Only (Capture)

VDAC3 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

230451C7

Cat no : 82666-14-PBS

Synonyms

VDAC-3, VDAC 3, hVDAC3, HD VDAC3, 230451C7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VDAC3 Recombinant antibody (82666-14-RR)

    WB analysis using 82666-14-RR (same clone as 82666-14-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 82666-14-RR (VDAC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82666-14-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293T cells using VDAC3 Recombinant antibody (82666-14-RR)

    WB analysis of HEK-293T using 82666-14-RR (same clone as 82666-14-PBS)

    WB result of VDAC3 antibody (82666-14-RR; 1:4000; room temperature for 1.5 hours) with wild-type and VDAC3 knockout HEK-293T cells. This data was developed using the same antibody clone with 82666-14-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using VDAC3 Recombinant antibody (82666-14-RR)

    IHC staining of human ovary cancer using 82666-14-RR (same clone as 82666-14-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82666-14-RR (VDAC3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82666-14-PBS in a different storage buffer formulation.

  • Cytometric bead array standard curve of MP00287-1

    Cytometric bead array standard curve of MP00287-1

    Cytometric bead array standard curve of MP00287-1, VDAC3 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 82666-14-PBS. Detection antibody: 82666-15-PBS. Standard: SY01307. Range: 0.156-10 ng/mL.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

82666-14-PBS targets VDAC3 as part of a matched antibody pair:

MP00287-1: 82666-14-PBS capture and 82666-15-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name voltage-dependent anion channel 3
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberNM_005662
Gene symbol VDAC3
Gene ID (NCBI) 7419
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC3.