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  • Featured Product
  • KD/KO Validated

VDAC3 Recombinant antibody

VDAC3 Recombinant Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

230451C7

Cat no : 82666-14-RR

Synonyms

VDAC-3, VDAC 3, hVDAC3, HD VDAC3, 230451C7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VDAC3 Recombinant antibody (82666-14-RR)

    WB analysis using 82666-14-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 82666-14-RR (VDAC3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HEK-293T cells using VDAC3 Recombinant antibody (82666-14-RR)

    WB analysis of HEK-293T using 82666-14-RR

    WB result of VDAC3 antibody (82666-14-RR; 1:4000; room temperature for 1.5 hours) with wild-type and VDAC3 knockout HEK-293T cells.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using VDAC3 Recombinant antibody (82666-14-RR)

    IHC staining of human ovary cancer using 82666-14-RR

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 82666-14-RR (VDAC3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inHepG2 cells, HEK-293T cells, NIH/3T3 cells, HeLa cells, A431 cells, mouse heart tissue, mouse testis tissue
Positive IHC detected inhuman ovary cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

82666-14-RR targets VDAC3 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name voltage-dependent anion channel 3
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberNM_005662
Gene symbol VDAC3
Gene ID (NCBI) 7419
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC3.

Protocols

Product Specific Protocols
WB protocol for VDAC3 antibody 82666-14-RRDownload protocol
IHC protocol for VDAC3 antibody 82666-14-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols