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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 750-conjugated VDAC3 Recombinant antibody

VDAC3 Recombinant Antibody for WB

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB

Conjugate

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

230451C7

Cat no : CL750-82666-14

Synonyms

VDAC-3, VDAC 3, hVDAC3, HD VDAC3, 230451C7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 750-conjugated VDAC3 Recombinant an (CL750-82666-14)

    WB analysis using CL750-82666-14

    Various lysates were subjected to SDS PAGE followed by western blot with CL750-82666-14 (VDAC3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 750-conjugated VDAC3 Recombinant an (CL750-82666-14)

    FC experiment of HeLa using CL750-82666-14

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated VDAC3 Recombinant antibody (CL750-82666-14, Clone:230451C7)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inA431 cells, HeLa cells，
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL750-82666-14 targets VDAC3 in WB applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name voltage-dependent anion channel 3
Calculated molecular weight 31 kDa
Observed molecular weight 31 kDa
GenBank accession numberNM_005662
Gene symbol VDAC3
Gene ID (NCBI) 7419
Conjugate CoraLite® Plus 750 Fluorescent Dye
Excitation/Emission maxima wavelengths755 nm / 780 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VDAC3 belongs to the eukaryotic mitochondrial porin family. It forms a channel through the mitochondrial outer membrane that allows diffusion of small hydrophilic molecules. This antibody is specific to VDAC3.

Protocols

Product Specific Protocols
WB protocol for CL Plus 750 VDAC3 antibody CL750-82666-14Download protocol
Standard Protocols
Click here to view our Standard Protocols