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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VE-cadherin/CD144 Monoclonal antibody, PBS Only

VE-cadherin/CD144 Monoclonal Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

4F9B10

Cat no : 66804-1-PBS

Synonyms

VE-cadherin, CDH5, CD144, Cadherin 5, 7B4 antigen

Validation Data Gallery

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  • Western Blot (WB) analysis of human placenta tissue using VE-cadherin/CD144 Monoclonal antibody (66804-1-Ig)

    WB analysis of human placenta using 66804-1-Ig (same clone as 66804-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 66804-1-Ig (VE-cadherin antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VE-cadherin/CD144 Monoclonal antibody (66804-1-Ig)

    IHC staining of human breast cancer using 66804-1-Ig (same clone as 66804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using VE-cadherin/CD144 Monoclonal antibody (66804-1-Ig)

    IHC staining of human breast cancer using 66804-1-Ig (same clone as 66804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VE-cadherin/CD144 Monoclonal antibody (66804-1-Ig)

    IHC staining of human tonsillitis using 66804-1-Ig (same clone as 66804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VE-cadherin/CD144 Monoclonal antibody (66804-1-Ig)

    IHC staining of human tonsillitis using 66804-1-Ig (same clone as 66804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:400 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VE-cadherin/CD144 Monoclonal antibody (66804-1-Ig)

    IHC staining of human placenta using 66804-1-Ig (same clone as 66804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VE-cadherin/CD144 Monoclonal antibody (66804-1-Ig)

    IHC staining of human placenta using 66804-1-Ig (same clone as 66804-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 66804-1-Ig (VE-cadherin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using VE-cadherin/CD144 Monoclonal antibody (66804-1-Ig)

    IF Staining of human placenta using 66804-1-Ig (same clone as 66804-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human placenta tissue using VE-cadherin antibody (66804-1-Ig, Clone: 4F9B10 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HUVEC cells using VE-cadherin/CD144 Monoclonal antibody (66804-1-Ig)

    IF Staining of HUVEC using 66804-1-Ig (same clone as 66804-1-PBS)

    Immunofluorescent analysis of un-fixed HUVEC cells using VE-cadherin/CD144 antibody (66804-1-Ig, Clone: 4F9B10 ) at dilution of 1:1500 and Multi-rAb CoraLite® Plus 594-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (Cat.NO. RGAM004 ). This data was developed using the same antibody clone with 66804-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66804-1-PBS targets VE-cadherin/CD144 in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VE-cadherin/CD144 fusion protein Ag27501 相同性解析による交差性が予測される生物種
Full Name cadherin 5, type 2 (vascular endothelium)
Calculated molecular weight 88 kDa
Observed molecular weight125 kDa, 100 kDa
GenBank accession numberNM_001795
Gene symbol VE-cadherin
Gene ID (NCBI) 1003
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Cadherins are a family of transmembrane glycoproteins that mediate calcium-dependent cell-cell adhesion and play an important role in the maintenance of normal tissue architecture. Vascular endothelial cadherin (VE-cadherin), also known as Cadherin-5 (CDH5) or CD144, is a member of the type II classical cadherin family of cell adhesion proteins (PMID: 21269602). VE-cadherin is expressed specifically in endothelial cells and mediates homophilic adhesion in the vascular endothelium (PMID: 1522121; 8555485; 21269602). VE-cadherin plays a role in the organization of lateral endothelial junctions and in the control of permeability properties of vascular endothelium (PMID: 1522121). VE-cadherin has also been shown to be required for angiogenesis (PMID: 16473763; 18162609).