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KD/KO Validated

VEGFA Monoclonal antibody

Name: VEGFA Monoclonal antibody
Brand: Proteintech
SKU: 66828-1-Ig

VEGFA Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human and More (4)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Conjugate

Unconjugated

Publications

110

CloneNo.

2E2H9

Review Product

Cat no : 66828-1-Ig

Synonyms

VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF

Validation Data Gallery

Filter:

AllWBIHCIF-PIF/ICCFC (Intra)

WB analysis of MCF-7 using 66828-1-Ig
MCF-7 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of SH-SY5Y using 66828-1-Ig
SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HuH-7 using 66828-1-Ig
HuH-7 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
WB analysis of T-47D using 66828-1-Ig
T-47D cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of MDA-MB-231 using 66828-1-Ig
MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of HUVEC using 66828-1-Ig
HUVEC cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of NCI-H1299 using 66828-1-Ig
NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
WB analysis of A431 using 66828-1-Ig
A431 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.
IHC staining of human lung cancer using 66828-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 66828-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 66828-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 66828-1-Ig
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 66828-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human colon cancer using 66828-1-Ig
Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 66828-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 66828-1-Ig
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of human endometrial cancer using 66828-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human endometrial cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human endometrial cancer using 66828-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human endometrial cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human lung cancer using 66828-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of human lung cancer using 66828-1-Ig
Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
IF Staining of MCF-7 using 66828-1-Ig
Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC experiment of MCF-7 using 66828-1-Ig
1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human VEGFA (66828-1-Ig, Clone:2E2H9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Cat No. 66828-1-Ig

Size/Concentration

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected in	MCF-7 cells, HUVEC cells, NCI-H1299 cells, A431 cells, SH-SY5Y cells, HuH-7 cells, T-47D cells, MDA-MB-231 cells
Positive IHC detected in	human lung cancer tissue, human liver cancer tissue, human colon cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF-P detected in	human endometrial cancer tissue, human lung cancer tissue
Positive IF/ICC detected in	MCF-7 cells
Positive FC (Intra) detected in	MCF-7 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:1000-1:4000
Immunohistochemistry (IHC)	IHC : 1:400-1:1600
Immunofluorescence (IF)-P	IF-P : 1:200-1:800
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 3 publications below
WB	See 95 publications below
IHC	See 18 publications below
IF	See 14 publications below
ELISA	See 1 publications below

Product Information

66828-1-Ig targets VEGFA in WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Cited Reactivity	human, rat, pig, zebrafish, goat
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	VEGFA fusion protein Ag13500 相同性解析による交差性が予測される生物種
Full Name	vascular endothelial growth factor A
Calculated molecular weight	412 aa, 46 kDa
Observed molecular weight	43 kDa
GenBank accession number	BC065522
Gene symbol	VEGFA
Gene ID (NCBI)	7422
RRID	AB_2882171
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.

Protocols

Product Specific Protocols
WB protocol for VEGFA antibody 66828-1-Ig	Download protocol
IHC protocol for VEGFA antibody 66828-1-Ig	Download protocol
IF protocol for VEGFA antibody 66828-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
	WB	Adv Sci (Weinh) Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution Authors - Taifeng Zhou View Article
pig	WB	Redox Biol Nox2 impairs VEGF-A-induced angiogenesis in placenta via mitochondrial ROS-STAT3 pathway. Authors - Chengjun Hu KD Validated View Article
human	WB	J Transl Med KPNA2 promotes angiogenesis by regulating STAT3 phosphorylation Authors - Yujie Jia View Article
human	WB	Oxid Med Cell Longev Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway. Authors - Ziwei Yang View Article
human	WB	Mol Ther Nucleic Acids circ_2858 Helps Blood-Brain Barrier Disruption by Increasing VEGFA via Sponging miR-93-5p during Escherichia coli Meningitis. Authors - Ruicheng Yang View Article
human	WB	Bioengineered LEM domain containing 1 (LEMD1) transcriptionally activated by SRY-related high-mobility-group box 4 (SOX4) accelerates the progression of colon cancer by upregulating phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway Authors - Ding Li View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VEGFA Monoclonal antibody

VEGFA Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), ELISA

Validation Data Gallery

WB analysis of MCF-7 using 66828-1-Ig

WB analysis of SH-SY5Y using 66828-1-Ig

WB analysis of HuH-7 using 66828-1-Ig

WB analysis of T-47D using 66828-1-Ig

WB analysis of MDA-MB-231 using 66828-1-Ig

WB analysis of HUVEC using 66828-1-Ig

WB analysis of NCI-H1299 using 66828-1-Ig

WB analysis of A431 using 66828-1-Ig

IHC staining of human lung cancer using 66828-1-Ig

IHC staining of human lung cancer using 66828-1-Ig

IHC staining of human lung cancer using 66828-1-Ig

IHC staining of human lung cancer using 66828-1-Ig

IHC staining of human colon cancer using 66828-1-Ig

IHC staining of human colon cancer using 66828-1-Ig

IHC staining of human liver cancer using 66828-1-Ig

IHC staining of human liver cancer using 66828-1-Ig

IF Staining of human endometrial cancer using 66828-1-Ig

IF Staining of human endometrial cancer using 66828-1-Ig

IF Staining of human lung cancer using 66828-1-Ig

IF Staining of human lung cancer using 66828-1-Ig

IF Staining of MCF-7 using 66828-1-Ig

FC experiment of MCF-7 using 66828-1-Ig

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Spatiotemporal Characterization of Human Early Intervertebral Disc Formation at Single-Cell Resolution

Nox2 impairs VEGF-A-induced angiogenesis in placenta via mitochondrial ROS-STAT3 pathway.

KPNA2 promotes angiogenesis by regulating STAT3 phosphorylation

Chitosan Oligosaccharides Alleviate H2O2-stimulated Granulosa Cell Damage via HIF-1α Signaling Pathway.

circ_2858 Helps Blood-Brain Barrier Disruption by Increasing VEGFA via Sponging miR-93-5p during Escherichia coli Meningitis.

LEM domain containing 1 (LEMD1) transcriptionally activated by SRY-related high-mobility-group box 4 (SOX4) accelerates the progression of colon cancer by upregulating phosphatidylinositol 3-kinase (PI3K)/protein kinase B (Akt) signaling pathway