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  • Featured Product
  • KD/KO Validated

VEGFA Monoclonal antibody, PBS Only

VEGFA Monoclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

2E2H9

Cat no : 66828-1-PBS

Synonyms

VEGF, Vascular endothelial growth factor A, long form, vascular endothelial growth factor A, Vascular Endothelial Growth Factor, N-VEGF

Validation Data Gallery

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  • Western Blot (WB) analysis of MCF-7 cells using VEGFA Monoclonal antibody (66828-1-Ig)

    WB analysis of MCF-7 using 66828-1-Ig (same clone as 66828-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of SH-SY5Y cells using VEGFA Monoclonal antibody (66828-1-Ig)

    WB analysis of SH-SY5Y using 66828-1-Ig (same clone as 66828-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HUVEC cells using VEGFA Monoclonal antibody (66828-1-Ig)

    WB analysis of HUVEC using 66828-1-Ig (same clone as 66828-1-PBS)

    HUVEC cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of NCI-H1299 cells using VEGFA Monoclonal antibody (66828-1-Ig)

    WB analysis of NCI-H1299 using 66828-1-Ig (same clone as 66828-1-PBS)

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HuH-7 cells using VEGFA Monoclonal antibody (66828-1-Ig)

    WB analysis of HuH-7 using 66828-1-Ig (same clone as 66828-1-PBS)

    HuH-7 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of T-47D cells using VEGFA Monoclonal antibody (66828-1-Ig)

    WB analysis of T-47D using 66828-1-Ig (same clone as 66828-1-PBS)

    T-47D cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MDA-MB-231 cells using VEGFA Monoclonal antibody (66828-1-Ig)

    WB analysis of MDA-MB-231 using 66828-1-Ig (same clone as 66828-1-PBS)

    MDA-MB-231 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A431 cells using VEGFA Monoclonal antibody (66828-1-Ig)

    WB analysis of A431 using 66828-1-Ig (same clone as 66828-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 66828-1-Ig (VEGFA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VEGFA Monoclonal antibody (66828-1-Ig)

    IHC staining of human lung cancer using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VEGFA Monoclonal antibody (66828-1-Ig)

    IHC staining of human lung cancer using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VEGFA Monoclonal antibody (66828-1-Ig)

    IHC staining of human lung cancer using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VEGFA Monoclonal antibody (66828-1-Ig)

    IHC staining of human colon cancer using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using VEGFA Monoclonal antibody (66828-1-Ig)

    IHC staining of human colon cancer using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using VEGFA Monoclonal antibody (66828-1-Ig)

    IHC staining of human lung cancer using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VEGFA Monoclonal antibody (66828-1-Ig)

    IHC staining of human liver cancer using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using VEGFA Monoclonal antibody (66828-1-Ig)

    IHC staining of human liver cancer using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 66828-1-Ig (VEGFA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human endometrial cancer tissue using VEGFA Monoclonal antibody (66828-1-Ig)

    IF Staining of human endometrial cancer using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human endometrial cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human endometrial cancer tissue using VEGFA Monoclonal antibody (66828-1-Ig)

    IF Staining of human endometrial cancer using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human endometrial cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using VEGFA Monoclonal antibody (66828-1-Ig)

    IF Staining of human lung cancer using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using VEGFA Monoclonal antibody (66828-1-Ig)

    IF Staining of human lung cancer using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using VEGFA Monoclonal antibody (66828-1-Ig)

    IF Staining of MCF-7 using 66828-1-Ig (same clone as 66828-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed MCF-7 cells using VEGFA antibody (66828-1-Ig, Clone: 2E2H9 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of MCF-7 cells using VEGFA Monoclonal antibody (66828-1-Ig)

    FC experiment of MCF-7 using 66828-1-Ig (same clone as 66828-1-PBS)

    1X10^6 MCF-7 cells were intracellularly stained with 0.5 ug Anti-Human VEGFA (66828-1-Ig, Clone:2E2H9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Mouse IgG2b Isotype Control (66360-3-Ig, Clone: K11B8C4B5) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66828-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66828-1-PBS targets VEGFA in WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen VEGFA fusion protein Ag13500 相同性解析による交差性が予測される生物種
Full Name vascular endothelial growth factor A
Calculated molecular weight 412 aa, 46 kDa
Observed molecular weight 43 kDa
GenBank accession numberBC065522
Gene symbol VEGFA
Gene ID (NCBI) 7422
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VEGFA, also named as VEGF or VPF, belongs to the PDGF/VEGF growth factor family. It is a growth factor active in angiogenesis, vasculogenesis and endothelial cell growth. VEGFA induces endothelial cell proliferation, promotes cell migration, inhibits apoptosis and induces permeabilization of blood vessels. It binds to the FLT1/VEGFR1 and KDR/VEGFR2 receptors, heparan sulfate and heparin. Defects in VEGFA are associated with microvascular complications of diabetes type 1 (MVCD1). VEGFA has 17 isoforms with MW from 16 to 45 kDa. Some isoforms have homodimer forms (e.g.; VEGFA189 38kDa or VEFGA110 34 kDa). VEGF-A exists in at least seven homodimeric isoforms. The monomers consist of 121, 145, 148, 165, 183, 189, or 206 amino acids (PMID:15602010 ). This antibody can recognize all VEGFA isoforms.