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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated VEGF-C Monoclonal antibody

VEGF-C Monoclonal Antibody for IF/ICC, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC, FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2B11F7

Cat no : CL488-67116

Synonyms

VEGFC, VEGF C, Vascular endothelial growth factor-related protein, vascular endothelial growth factor C, 2B11F7

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using CoraLite® Plus 488-conjugated VEGFC Monoclonal ant (CL488-67116)

    IF Staining of MCF-7 using CL488-67116

    Immunofluorescent analysis of (-20°C Methanol) fixed MCF-7 cells using CoraLite® Plus 488 VEGFC antibody (CL488-67116, Clone: 2B11F7 ) at dilution of 1:200.

  • Flow cytometry (FC) experiment of MCF-7 cells using CoraLite® Plus 488-conjugated VEGFC Monoclonal ant (CL488-67116)

    FC experiment of MCF-7 using CL488-67116

    1x10^6 MCF-7 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488-conjugated VEGFC Monoclonal antibody (CL488-67116, Clone:2B11F7)(red), or 0.4 ug CoraLite® Plus 488 Mouse IgG1 Isotype Control (1F8D3) (CL488-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive IF/ICC detected inMCF-7 cells
Positive FC (Intra) detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67116 targets VEGF-C in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VEGF-C fusion protein Ag18182 相同性解析による交差性が予測される生物種
Full Name vascular endothelial growth factor C
Calculated molecular weight 419 aa, 47 kDa
Observed molecular weight 47 kDa
GenBank accession numberBC035212
Gene symbol VEGFC
Gene ID (NCBI) 7424
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VEGFC is a member of the platelet-derived growth factor / vascular endothelial growth factor (PDGF/VEGF) family. The main function of VEGFC is to promote the growth of lymphatic vessels (lymphangiogenesis). It acts on lymphatic endothelial cells (LECs) primarily via its receptor VEGFR-3 promoting survival, growth, and migration.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 VEGF-C antibody CL488-67116Download protocol
FC protocol for CL Plus 488 VEGF-C antibody CL488-67116Download protocol
Standard Protocols
Click here to view our Standard Protocols