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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated VGLUT1 Polyclonal an (CL488-55491)

    IF Staining of mouse brain using CL488-55491

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CoraLite® Plus 488 VGLUT1 antibody (CL488-55491) at dilution of 1:200.

  • Flow cytometry (FC) experiment of SH-SY5Y cells using CoraLite® Plus 488-conjugated VGLUT1 Polyclonal an (CL488-55491)

    FC experiment of SH-SY5Y using CL488-55491

    1X10^6 SH-SY5Y cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human VGLUT1 (CL488-55491) (red), or 0.8 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite® Plus 488-conjugated VGLUT1 Polyclonal antibody

VGLUT1 Polyclonal Antibody for IF-P, FC (Intra)
Cat No. CL488-55491

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P, FC (Intra)

Synonyms

1, SLC17A7, BNPI, Brain-specific Na(+)-dependent inorganic phosphate cotransporter

Formulation:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF-P detected inmouse brain tissue
Positive FC (Intra) detected inSH-SY5Y cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-55491 targets VGLUT1 in IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name solute carrier family 17 (sodium-dependent inorganic phosphate cotransporter), member 7
Calculated molecular weight 62 kDa
GenBank accession numberNM_020309
Gene Symbol VGLUT1
Gene ID (NCBI) 57030
RRIDAB_3084131
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
激发激光Blue laser (488 nm)
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9P2U7
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vesicular glutamate transporter 1 (VGLUT1) is a critical protein involved in the packaging and release of the neurotransmitter glutamate in synaptic vesicles. VGLUT1 is predominantly expressed in neurons that release glutamate, the primary excitatory neurotransmitter in the central nervous system, it is a marker of glutamatergic neurons.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 VGLUT1 antibody CL488-55491Download protocol
IF protocol for CL Plus 488 VGLUT1 antibody CL488-55491Download protocol
Standard Protocols
Click here to view our Standard Protocols
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