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  • Featured Product
  • KD/KO Validated

Vimentin Polyclonal antibody

Vimentin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment

Conjugate

Unconjugated

Cat no : 10366-1-AP

Synonyms

VIM

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using Vimentin Polyclonal antibody (10366-1-AP)

    WB analysis using 10366-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10366-1-AP (Vimentin antibody) at dilution of 1:50000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using Vimentin Polyclonal antibody (10366-1-AP)

    WB analysis of Jurkat cells using 10366-1-AP

    WB result of Vimentin antibody (10366-1-AP, 1:4000) with si-Control and si-Vimentin transfected Jurkat cells.

  • Western Blot (WB) analysis of NIH/3T3 cells using Vimentin Polyclonal antibody (10366-1-AP)

    WB analysis of NIH/3T3 using 10366-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10366-1-AP (VIM antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using Vimentin Polyclonal antibody (10366-1-AP)

    WB analysis of mouse heart using 10366-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 10366-1-AP (VIM Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IHC staining of human liver cancer using 10366-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IHC staining of human hepatocirrhosis using 10366-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:5000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IHC staining of human hepatocirrhosis using 10366-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:10000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IHC staining of human hepatocirrhosis using 10366-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:10000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IHC staining of human colon using 10366-1-AP

    Immunohistochemical analysis of paraffin-embedded normal colon slide using 10366-1-AP (Vimentin antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IHC staining of human breast cancer using 10366-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:3000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IHC staining of human liver using 10366-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver using 10366-1-AP (VIM antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IHC staining of human tonsillitis using 10366-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IHC staining of human tonsillitis using 10366-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 10366-1-AP (Vimentin antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IF Staining of human breast cancer using 10366-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IF Staining of human kidney using 10366-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human breast cancer tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IF Staining of human breast cancer using 10366-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human kidney tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IF Staining of human kidney using 10366-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human kidney tissue using Vimentin antibody (10366-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using Vimentin Polyclonal antibody (10366-1-AP)

    IF Staining of mouse brain using 10366-1-AP

    Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse brain tissue using 10366-1-AP (Vimentin antibody) at dilution of 1:400 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Vimentin Polyclonal antibody (10366-1-AP)

    IF Staining of HepG2 using 10366-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10366-1-AP (Vimentin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Vimentin Polyclonal antibody (10366-1-AP)

    IF Staining of HepG2 using 10366-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Vimentin antibody (10366-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Vimentin Polyclonal antibody (10366-1-AP)

    IF Staining of HepG2 using 10366-1-AP

    Immunofluorescent analysis of HepG2 cells, using VIM antibody 10366-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  • Flow cytometry (FC) experiment of HeLa cells using Vimentin Polyclonal antibody (10366-1-AP)

    FC experiment of HeLa using 10366-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Vimentin (10366-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Flow cytometry (FC) experiment of mMSCs cells using Vimentin Polyclonal antibody (10366-1-AP)

    FC experiment of mMSCs using 10366-1-AP

    1x10^6 mMSCs cells were intracellularly stained with 0.5 ug Vimentin Polyclonal antibody (10366-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.5 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive WB detected inHepG2 cells, NIH/3T3 cells, mouse heart tissue, Jurkat cells, HeLa cells
Positive IHC detected inhuman liver cancer tissue, human breast cancer tissue, human hepatocirrhosis tissue, human liver tissue, human normal colon, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman breast cancer tissue, human kidney tissue
Positive IF-Fro detected inmouse brain tissue
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells, mMSCs cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)-FROIF-FRO : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10366-1-AP targets Vimentin in WB, IHC, IF/ICC, IF-P, IF-Fro, FC (Intra), IP, CoIP, RIP, ELISA, Cell treatment applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, bovine, hamster, sheep, goat, deer
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vimentin fusion protein Ag0489 相同性解析による交差性が予測される生物種
Full Name vimentin
Calculated molecular weight 466 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
RRIDAB_2273020
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Protocols

Product Specific Protocols
WB protocol for Vimentin antibody 10366-1-APDownload protocol
IHC protocol for Vimentin antibody 10366-1-APDownload protocol
IF protocol for Vimentin antibody 10366-1-APDownload protocol
FC protocol for Vimentin antibody 10366-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IF

Nat Cancer

Spatial single-cell protein landscape reveals vimentinhigh macrophages as immune-suppressive in the microenvironment of hepatocellular carcinoma

Authors - Xinyao Qiu
View Article
humanWB

Cell Res

The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.

Authors - Jia-Yuan Zhang
View Article
humanWB

ACS Nano

Deep-Learning-Based Nanomechanical Vibration for Rapid and Label-Free Assay of Epithelial Mesenchymal Transition

Authors - Wenjie Wu
View Article
mouseIF

Cell Host Microbe

Gut microbiome dysbiosis contributes to abdominal aortic aneurysm by promoting neutrophil extracellular trap formation

Authors - Zhenyu Tian
View Article
human

Adv Sci (Weinh)

PMID: 31728273

Authors - Qingming Tang
View Article
IHC

Circ Res

Glutamyl-Prolyl-tRNA Synthetase Regulates Proline-Rich Pro-Fibrotic Protein Synthesis During Cardiac Fibrosis.

Authors - Jiangbin Wu
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

FH

Vernon (Verified Customer) (01-29-2024)

Western blot signal is great with 30 sec exposure secondary antibody 1:5000 1hr. at room temp

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Ewing sarcoma cell line
Vimentin Antibody Western Blot validation (1:2000 dilution) in Ewing sarcoma cell line (Cat no:10366-1-AP)
FH

Tobias (Verified Customer) (06-16-2023)

Works well in IF

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
FH

Emma (Verified Customer) (02-22-2022)

Works well by IF on FFPE prostate tissue. (Tris-EDTA antigen retrieval).

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Prostate
FH

Yuki (Verified Customer) (01-07-2022)

Consistently works antibody

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:150
  • Cell Tissue Type: gut duct tissue
Vimentin Antibody Immunofluorescence validation (1:150 dilution) in gut duct tissue (Cat no:10366-1-AP)
FH

Hala (Verified Customer) (07-08-2021)

Works veryyyy gooddd

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000 - 1/2000
  • Cell Tissue Type: hepG2 transduced with TDO2 gene, liver cancer cells
FH

Hala (Verified Customer) (04-11-2021)

very good antibody, works very well

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: hepG2 transduced with TDO2 gene
Vimentin Antibody Western Blot validation (1/1000 dilution) in hepG2 transduced with TDO2 gene (Cat no:10366-1-AP)
FH

wei (Verified Customer) (04-27-2020)

I tried in C2C12 cells

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: C2C12
Vimentin Antibody Western Blot validation (1:1000 dilution) in C2C12 (Cat no:10366-1-AP)
FH

Patricia (Verified Customer) (02-25-2020)

Works well.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Ovarian cancer
FH

Kishor (Verified Customer) (01-30-2019)

Good results in western blotting but I could not see consistent results in IF

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: H69, HUCCT-1
FH

Jaime (Verified Customer) (01-24-2018)

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1/200
  • Cell Tissue Type: Mouse ventricle frozen sections
Vimentin Antibody Immunofluorescence validation (1/200 dilution) in Mouse ventricle frozen sections (Cat no:10366-1-AP)