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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated Vimentin Polyclonal antibody

Vimentin Polyclonal Antibody for FC (Intra), IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-10366

Synonyms

Cell and organelle markers, Cytoskeleton Marker, FLJ36605, VIM, vimentin

Validation Data Gallery

Applications:
  • IF Staining of HepG2 using CL594-10366

    IF Staining of HepG2 using CL594-10366

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 Vimentin antibody (CL594-10366) at dilution of 1:500.

  • IF Staining of HepG2 using CL594-10366

    IF Staining of HepG2 using CL594-10366

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 Vimentin antibody (CL594-10366) at dilution of 1:600. DAPI (blue).

  • FC experiment of HeLa using CL594-10366

    FC experiment of HeLa using CL594-10366

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human Vimentin (CL594-10366) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inHepG2 cells
Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 10366-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:250-1:1000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-10366 targets Vimentin in IF, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vimentin fusion protein Ag0489 相同性解析による交差性が予測される生物種
Full Name vimentin
Calculated molecular weight 466 aa, 54 kDa
Observed molecular weight 54 kDa
GenBank accession numberBC000163
Gene symbol VIM
Gene ID (NCBI) 7431
RRIDAB_2919730
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. Isoforms of vimentin (49 kDa and 60 kDa) had also been reported. (PMID: 8640945, 22728585).

Protocols

Product Specific Protocols
IF protocol for CL594 Vimentin antibody CL594-10366Download protocol
FC protocol for CL594 Vimentin antibody CL594-10366Download protocol
Standard Protocols
Click here to view our Standard Protocols