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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VISTA Recombinant antibody, PBS Only

VISTA Recombinant Antibody for WB, IHC, IF-P, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Conjugate

Unconjugated

CloneNo.

241003A4

Cat no : 83919-4-PBS

Synonyms

C10orf54, SISP1, PP2135, GI24, B7-H5

Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using VISTA Recombinant antibody (83919-4-RR)

    WB analysis of human peripheral blood platelets using 83919-4-RR (same clone as 83919-4-PBS)

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 83919-4-RR (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VISTA Recombinant antibody (83919-4-RR)

    IHC staining of human placenta using 83919-4-RR (same clone as 83919-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VISTA Recombinant antibody (83919-4-RR)

    IHC staining of human placenta using 83919-4-RR (same clone as 83919-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VISTA Recombinant antibody (83919-4-RR)

    IHC staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VISTA Recombinant antibody (83919-4-RR)

    IHC staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using VISTA Recombinant antibody (83919-4-RR)

    IF Staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using VISTA Recombinant antibody (83919-4-RR)

    IF Staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using VISTA Recombinant antibody (83919-4-RR)

    IF Staining of human tonsillitis using 83919-4-RR (same clone as 83919-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83919-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83919-4-RR

    Affinity and Kinetic Characterization of 83919-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83919-4-RR against Human VISTA were performed. The affinity constant is 57.9 nM.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83919-4-PBS is VISTA Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Fusion Protein 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 54
Calculated molecular weight 311 aa, 34 kDa
Observed molecular weight50 kDa
GenBank accession numberBC020568
Gene symbol VISTA
Gene ID (NCBI) 64115
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.