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Validation Data Gallery

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  • Western Blot (WB) analysis of human peripheral blood platelets using VISTA Recombinant antibody (83919-4-RR)

    WB analysis of human peripheral blood platelets using 83919-4-RR

    human peripheral blood platelets were subjected to SDS PAGE followed by western blot with 83919-4-RR (VISTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human placenta tissue using VISTA Recombinant antibody (83919-4-RR)

    IHC staining of human placenta using 83919-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human placenta tissue using VISTA Recombinant antibody (83919-4-RR)

    IHC staining of human placenta using 83919-4-RR

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VISTA Recombinant antibody (83919-4-RR)

    IHC staining of human tonsillitis using 83919-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VISTA Recombinant antibody (83919-4-RR)

    IHC staining of human tonsillitis using 83919-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using VISTA Recombinant antibody (83919-4-RR)

    IHC staining of human tonsillitis using 83919-4-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83919-4-RR (VISTA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using VISTA Recombinant antibody (83919-4-RR)

    IF Staining of human tonsillitis using 83919-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using VISTA Recombinant antibody (83919-4-RR)

    IF Staining of human tonsillitis using 83919-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human placenta tissue using VISTA Recombinant antibody (83919-4-RR)

    IF Staining of human placenta using 83919-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using VISTA Recombinant antibody (83919-4-RR)

    IF Staining of human tonsillitis using 83919-4-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human tonsillitis tissue using VISTA antibody (83919-4-RR, Clone: 241003A4 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Affinity and Kinetic Characterization of 83919-4-RR

    Affinity and Kinetic Characterization of 83919-4-RR

    Biolayer interferometry (BLl) kinetic assays of 83919-4-RR against Human VISTA were performed. The affinity constant is 57.9 nM.

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VISTA Recombinant antibody

VISTA Uni-rAbTM Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 83919-4-RR
Clone No.241003A4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF-P, ELISA

Synonyms

C10orf54, SISP1, PP2135, GI24, B7-H5

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
CoraLite®532
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inhuman peripheral blood platelets
Positive IHC detected inhuman placenta tissue, human tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman tonsillitis tissue, human placenta tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83919-4-RR targets VISTA in WB, IHC, IF-P, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name chromosome 10 open reading frame 54
Calculated molecular weight 311 aa, 34 kDa
Observed molecular weight50 kDa
GenBank accession numberBC020568
Gene Symbol VISTA
Gene ID (NCBI) 64115
RRIDAB_3671499
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ9H7M9
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VISTA, also known as platelet receptor Gi24, B7-H5, C10orf54, Dies1, PD-1H, and SISP1, is a member of the immunoglobulin superfamily. It serves not only as a positive regulator but also as a substrate of MT1-MMP and contributes at least in part to tumor invasion.

Protocols

Product Specific Protocols
WB protocol for VISTA antibody 83919-4-RRDownload protocol
IHC protocol for VISTA antibody 83919-4-RRDownload protocol
IF protocol for VISTA antibody 83919-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols