Validation Data Gallery
Tested Applications
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-10236 targets VPS35 in IF/ICC applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | VPS35 fusion protein Ag0340 相同性解析による交差性が予測される生物種 |
| Full Name | vacuolar protein sorting 35 homolog (S. cerevisiae) |
| Calculated molecular weight | 92 kDa |
| Observed molecular weight | 80-92 kDa |
| GenBank accession number | BC002414 |
| Gene Symbol | VPS35 |
| Gene ID (NCBI) | 55737 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96QK1 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VPS35 protein belongs to a group of vacuolar protein sorting (VPS) proteins, which ensure the proper delivery of organelle-specific proteins in eukaryotic cells. VPS35 is the core of a multimeric complex, termed the retromer complex, which is involved in the retrograde transport of proteins from endosomes to the trans-Golgi network. Vps35 serves as the core of the multimeric complex by binding directly to Vps26 and Vps29 and SNX1. Northern blot analyses in 16 tissues showed that one transcript of Vps35 with a size of 3.6 kb was highly expressed in the brain, heart, testis, ovary, small intestine, spleen, skeletal muscle, and placenta and expressed at moderate or low levels in other tissues. Another transcript of Vps35, a message of 3.0 kb, was also expressed proportionally lower levels than the 3.6-kb transcript in all the tissues except that the 3.0-kb transcript was not detected in the brain. Human Vps35 is mapped at 16q13-q21.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 VPS35 antibody CL488-10236 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |