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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS45 Polyclonal antibody, PBS Only

VPS45 Polyclonal Antibody for IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 12006-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS45 Polyclonal antibody (12006-1-AP)

    IHC staining of mouse brain using 12006-1-AP (same clone as 12006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using VPS45 Polyclonal antibody (12006-1-AP)

    IHC staining of mouse brain using 12006-1-AP (same clone as 12006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12006-1-AP (VPS45 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using VPS45 Polyclonal antibody (12006-1-AP)

    IHC staining of human heart using 12006-1-AP (same clone as 12006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using VPS45 Polyclonal antibody (12006-1-AP)

    IHC staining of human testis using 12006-1-AP (same clone as 12006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using VPS45 Polyclonal antibody (12006-1-AP)

    IHC staining of human testis using 12006-1-AP (same clone as 12006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human heart tissue using VPS45 Polyclonal antibody (12006-1-AP)

    IHC staining of human heart using 12006-1-AP (same clone as 12006-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human heart using 12006-1-AP (VPS45 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VPS45 Polyclonal antibody (12006-1-AP)

    IF Staining of HeLa using 12006-1-AP (same clone as 12006-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 12006-1-AP (VPS45 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VPS45 Polyclonal antibody (12006-1-AP)

    IF Staining of HepG2 using 12006-1-AP (same clone as 12006-1-PBS)

    Immunofluorescent analysis of HepG2 cells, using VPS45 antibody 12006-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye). This data was developed using the same antibody clone with 12006-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

12006-1-PBS targets VPS45 in IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS45 fusion protein Ag2658 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 45 homolog (S. cerevisiae)
Calculated molecular weight 570 aa, 65 kDa
GenBank accession numberBC012932
Gene symbol VPS45
Gene ID (NCBI) 11311
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

The Sec1/Munc18 (SM) family of proteins regulate intracellular trafficking through interactions with individual SNARE (soluble N-ethylmeleimide-sensitive factor attachment protein receptor) proteins and assembled SNARE complexes. The SM protein VPS45 (Vacuolar protein sorting-associated protein 45) has a calculated molecular mass of 65 kDa and shares 38% identity with yeast Vps45 which is required for the formation of yeast endosomal SNARE complexes and is thus essential for traffic through the endosomal system. VPS45 may play a role in vesicle-mediated protein trafficking from the Golgi stack through the trans-Golgi network. (PMID: 8996080; 23166732)