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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS4B Monoclonal antibody

VPS4B Monoclonal Antibody for WB, IF/ICC, IP, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat

Applications

WB, IF/ICC, IP, ELISA

Conjugate

Unconjugated

CloneNo.

2H3G7

Cat no : 68443-1-Ig

Synonyms

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS4B Monoclonal antibody (68443-1-Ig)

    WB analysis using 68443-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of NIH/3T3 cells using VPS4B Monoclonal antibody (68443-1-Ig)

    WB analysis of NIH/3T3 using 68443-1-Ig

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 68443-1-Ig (VPS4B antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using VPS4B Monoclonal antibody (68443-1-Ig)

    IP experiment of HeLa using 68443-1-Ig

    IP result of anti-VPS4B (IP:68443-1-Ig, 4ug; Detection:68443-1-Ig 1:4000) with HeLa cells lysate 1560 ug.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using VPS4B Monoclonal antibody (68443-1-Ig)

    IF Staining of HeLa using 68443-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using VPS4B antibody (68443-1-Ig, Clone: 2H3G7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inLNCaP cells, NIH/3T3 cells, HeLa cells, HEK-293 cells, Jurkat cells, K-562 cells, HSC-T6 cells
Positive IP detected inHeLa cells
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

68443-1-Ig targets VPS4B in WB, IF/ICC, IP, ELISA applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VPS4B fusion protein Ag11610 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 4 homolog B (S. cerevisiae)
Calculated molecular weight 444 aa, 49 kDa
Observed molecular weight~50 kDa
GenBank accession numberBC039574
Gene symbol VPS4B
Gene ID (NCBI) 9525
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)

Protocols

Product Specific Protocols
WB protocol for VPS4B antibody 68443-1-IgDownload protocol
IF protocol for VPS4B antibody 68443-1-IgDownload protocol
IP protocol for VPS4B antibody 68443-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols