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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated VPS4B Monoclonal antibody

VPS4B Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse , rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2H3G7

Cat no : CL488-68443

Synonyms

MIG1, Protein SKD1, SKD1, VPS4 2, VPS42, VPS4B

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated VPS4B Monoclonal ant (CL488-68443)

    IF Staining of HeLa using CL488-68443

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using CoraLite® Plus 488 VPS4B antibody (CL488-68443, Clone: 2H3G7 ) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-68443 targets VPS4B in IF/ICC applications and shows reactivity with Human, mouse , rat samples.

Tested Reactivity Human, mouse , rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VPS4B fusion protein Ag11610 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 4 homolog B (S. cerevisiae)
Calculated molecular weight 444 aa, 49 kDa
Observed molecular weight~50 kDa
GenBank accession numberBC039574
Gene symbol VPS4B
Gene ID (NCBI) 9525
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 VPS4B antibody CL488-68443Download protocol
Standard Protocols
Click here to view our Standard Protocols