Validation Data Gallery
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Tested Applications
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL488-68443 targets VPS4B in IF/ICC applications and shows reactivity with Human, mouse , rat samples.
| Tested Reactivity | Human, mouse , rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | VPS4B fusion protein Ag11610 相同性解析による交差性が予測される生物種 |
| Full Name | vacuolar protein sorting 4 homolog B (S. cerevisiae) |
| Calculated molecular weight | 444 aa, 49 kDa |
| Observed molecular weight | ~50 kDa |
| GenBank accession number | BC039574 |
| Gene Symbol | VPS4B |
| Gene ID (NCBI) | 9525 |
| RRID | AB_3673016 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | O75351 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
VPS4 (Vacuolar protein sorting-associated protein 4) is involved in intracellular protein trafficking, presumably at a late endosomal protein transport step. VPS4B is a ubiquitously expressed type I AAA+ (ATPases Associated with diverse cellular Activities) ATPase. It cooperates with its highly homologous paralog VPS4A (encoded by the VPS4A gene) to disassemble and release the Endosomal Sorting Complex Required for Transport (ESCRT) machinery. 68443-1-Ig specifically recognizes VPS4B. (PMID:31930723)
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 VPS4B antibody CL488-68443 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |