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Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using VPS52 Polyclonal antibody (11662-2-AP)

    WB analysis of HepG2 using 11662-2-AP (same clone as 11662-2-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse skin tissue using VPS52 Polyclonal antibody (11662-2-AP)

    WB analysis of mouse skin using 11662-2-AP (same clone as 11662-2-PBS)

    mouse skin tissue were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using VPS52 Polyclonal antibody (11662-2-AP)

    WB analysis of COLO 320 using 11662-2-AP (same clone as 11662-2-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 11662-2-AP (VPS52 antibody) at dilution of 1:0 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse skin tissue using VPS52 Polyclonal antibody (11662-2-AP)

    IP experiment of mouse skin using 11662-2-AP (same clone as 11662-2-PBS)

    IP result of anti-VPS52 (IP:11662-2-AP, 3ug; Detection:11662-2-AP 1:1000) with mouse skin tissue lysate 3200ug. This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human gliomas tissue using VPS52 Polyclonal antibody (11662-2-AP)

    IHC staining of human gliomas using 11662-2-AP (same clone as 11662-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human gliomas using 11662-2-AP (VPS52 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using VPS52 Polyclonal antibody (11662-2-AP)

    IF Staining of HepG2 using 11662-2-AP (same clone as 11662-2-PBS)

    Immunofluorescent analysis of HepG2 cells using 11662-2-AP (VPS52 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 11662-2-PBS in a different storage buffer formulation.

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VPS52 Polyclonal antibody, PBS Only

VPS52 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 11662-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

11662-2-PBS targets VPS52 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen VPS52 fusion protein Ag2180 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 52 homolog (S. cerevisiae)
Calculated molecular weight 723 aa, 82 kDa
Observed molecular weight 82 kDa
GenBank accession numberBC032108
Gene Symbol VPS52
Gene ID (NCBI) 6293
RRIDAB_2241614
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ8N1B4
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

VPS52 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins. VPS52 may play a role in the development of a multicellular organism through cell-cell interactions (PMID: 23142660).