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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS53 Monoclonal antibody (67610-1-Ig)

    WB analysis using 67610-1-Ig (same clone as 67610-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control. This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VPS53 Monoclonal antibody (67610-1-Ig)

    WB analysis using 67610-1-Ig (same clone as 67610-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using VPS53 Monoclonal antibody (67610-1-Ig)

    WB analysis using 67610-1-Ig (same clone as 67610-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using VPS53 Monoclonal antibody (67610-1-Ig)

    IHC staining of human liver using 67610-1-Ig (same clone as 67610-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using VPS53 Monoclonal antibody (67610-1-Ig)

    IHC staining of human liver using 67610-1-Ig (same clone as 67610-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using VPS53 Monoclonal antibody (67610-1-Ig)

    IHC staining of rat brain using 67610-1-Ig (same clone as 67610-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using VPS53 Monoclonal antibody (67610-1-Ig)

    IHC staining of rat brain using 67610-1-Ig (same clone as 67610-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using VPS53 Monoclonal antibody (67610-1-Ig)

    IF Staining of HEK-293 using 67610-1-Ig (same clone as 67610-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67610-1-Ig (VPS53 antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67610-1-PBS in a different storage buffer formulation.

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VPS53 Monoclonal antibody, PBS Only

VPS53 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67610-1-PBS
Clone No.2B12C3

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

FLJ10979, HCCS1, hVps53L, pp13624, VPS53

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67610-1-PBS targets VPS53 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VPS53 fusion protein Ag29875 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 53 homolog (S. cerevisiae)
Calculated molecular weight 832 aa, 94 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC029560
Gene Symbol VPS53
Gene ID (NCBI) 55275
RRIDAB_2882816
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ5VIR6
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.