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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VPS53 Monoclonal antibody

VPS53 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

CloneNo.

2B12C3

Cat no : 67610-1-Ig

Synonyms

FLJ10979, HCCS1, hVps53L, pp13624, VPS53

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using VPS53 Monoclonal antibody (67610-1-Ig)

    WB analysis using 67610-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.

  • Western Blot (WB) analysis of various lysates using VPS53 Monoclonal antibody (67610-1-Ig)

    WB analysis using 67610-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using VPS53 Monoclonal antibody (67610-1-Ig)

    WB analysis using 67610-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67610-1-Ig (VPS53 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using VPS53 Monoclonal antibody (67610-1-Ig)

    IHC staining of human liver using 67610-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human liver tissue using VPS53 Monoclonal antibody (67610-1-Ig)

    IHC staining of human liver using 67610-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using VPS53 Monoclonal antibody (67610-1-Ig)

    IHC staining of rat brain using 67610-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of rat brain tissue using VPS53 Monoclonal antibody (67610-1-Ig)

    IHC staining of rat brain using 67610-1-Ig

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 67610-1-Ig (VPS53 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using VPS53 Monoclonal antibody (67610-1-Ig)

    IF Staining of HEK-293 using 67610-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using 67610-1-Ig (VPS53 antibody), at dilution of 1:300 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

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Tested Applications

Positive WB detected inU2OS cells, HeLa cells, NIH/3T3 cells, HEK-293 cells, HepG2 cells, Jurkat cells, K-562 cells, HSC-T6 cells, LNCaP cells, 4T1 cells
Positive IHC detected inhuman liver tissue, rat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunohistochemistry (IHC)IHC : 1:300-1:1200
Immunofluorescence (IF)/ICCIF/ICC : 1:150-1:600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67610-1-Ig targets VPS53 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VPS53 fusion protein Ag29875 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 53 homolog (S. cerevisiae)
Calculated molecular weight 832 aa, 94 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC029560
Gene symbol VPS53
Gene ID (NCBI) 55275
RRIDAB_2882816
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.

Protocols

Product Specific Protocols
WB protocol for VPS53 antibody 67610-1-IgDownload protocol
IHC protocol for VPS53 antibody 67610-1-IgDownload protocol
IF protocol for VPS53 antibody 67610-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols