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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®555-conjugated VPS53 Monoclonal antibody

VPS53 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

IF/ICC

Conjugate

CoraLite®555 Fluorescent Dye

CloneNo.

2B12C3

Cat no : CL555-67610

Synonyms

FLJ10979, HCCS1, hVps53L, pp13624, VPS53

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HEK-293 cells using CoraLite®555-conjugated VPS53 Monoclonal antibody (CL555-67610)

    IF Staining of HEK-293 using CL555-67610

    Immunofluorescent analysis of (-20°C Ethanol) fixed HEK-293 cells using CoraLite®555 VPS53 antibody (CL555-67610, Clone: 2B12C3 ) at dilution of 1:400.

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Tested Applications

Positive IF/ICC detected inHEK-293 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL555-67610 targets VPS53 in IF/ICC applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VPS53 fusion protein Ag29875 相同性解析による交差性が予測される生物種
Full Name vacuolar protein sorting 53 homolog (S. cerevisiae)
Calculated molecular weight 832 aa, 94 kDa
Observed molecular weight 94 kDa
GenBank accession numberBC029560
Gene symbol VPS53
Gene ID (NCBI) 55275
Conjugate CoraLite®555 Fluorescent Dye
Excitation/Emission maxima wavelengths557 nm / 570nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

VPS53 is a component of the Golgi-associated retrograde protein (GARP) complex, also called VFT (VPS fifty-three) complex, composed of VPS51, VPS52, VPS53 and VPS54. GARP complex functions in traffic from endosomes to the trans-Golgi network (PMID: 15878329). GARP proteins interact with RAB proteins and SNARE proteins.

Protocols

Product Specific Protocols
IF protocol for CL555 VPS53 antibody CL555-67610Download protocol
Standard Protocols
Click here to view our Standard Protocols