Validation Data Gallery
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Tested Applications
| Positive WB detected in | human plasma tissue, pig plasma tissue, rabbit serum tissue |
| Positive IHC detected in | human testis tissue, human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | NIH/3T3 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:10000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| IHC | See 1 publications below |
Product Information
66398-1-Ig targets VTN in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig, rabbit, mouse samples.
| Tested Reactivity | human, pig, rabbit, mouse |
| Cited Reactivity | pig |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | VTN fusion protein Ag8443 相同性解析による交差性が予測される生物種 |
| Full Name | vitronectin |
| Calculated molecular weight | 478 aa, 54 kDa |
| Observed molecular weight | 65 kDa |
| GenBank accession number | BC005046 |
| Gene Symbol | VTN |
| Gene ID (NCBI) | 7448 |
| RRID | AB_2881772 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P04004 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vitronectin is a glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for VTN antibody 66398-1-Ig | Download protocol |
| IHC protocol for VTN antibody 66398-1-Ig | Download protocol |
| IF protocol for VTN antibody 66398-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|