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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

VTN Monoclonal antibody, PBS Only

VTN Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Mouse / IgG1

Reactivity

human, pig, rabbit, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

1D2C8

Cat no : 66398-1-PBS

Synonyms

S protein, Serum spreading factor, V75, vitronectin, VN, VNT, VTN

Validation Data Gallery

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  • Western Blot (WB) analysis of human plasma using VTN Monoclonal antibody (66398-1-Ig)

    WB analysis of human plasma using 66398-1-Ig (same clone as 66398-1-PBS)

    human plasma were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of pig plasma using VTN Monoclonal antibody (66398-1-Ig)

    WB analysis of pig plasma using 66398-1-Ig (same clone as 66398-1-PBS)

    pig plasma were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rabbit serum using VTN Monoclonal antibody (66398-1-Ig)

    WB analysis of rabbit serum using 66398-1-Ig (same clone as 66398-1-PBS)

    rabbit serum were subjected to SDS PAGE followed by western blot with 66398-1-Ig (VTN Antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using VTN Monoclonal antibody (66398-1-Ig)

    IHC staining of human testis using 66398-1-Ig (same clone as 66398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:350 (under 10x lens). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using VTN Monoclonal antibody (66398-1-Ig)

    IHC staining of human testis using 66398-1-Ig (same clone as 66398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using VTN Monoclonal antibody (66398-1-Ig)

    IHC staining of human liver using 66398-1-Ig (same clone as 66398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using VTN Monoclonal antibody (66398-1-Ig)

    IHC staining of human liver using 66398-1-Ig (same clone as 66398-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 66398-1-Ig (VTN Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of NIH/3T3 cells using VTN Monoclonal antibody (66398-1-Ig)

    IF Staining of NIH/3T3 using 66398-1-Ig (same clone as 66398-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using VTN antibody (66398-1-Ig, Clone: 1D2C8 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66398-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66398-1-PBS targets VTN in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig, rabbit, mouse samples.

Tested Reactivity human, pig, rabbit, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen VTN fusion protein Ag8443 相同性解析による交差性が予測される生物種
Full Name vitronectin
Calculated molecular weight 478 aa, 54 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC005046
Gene symbol VTN
Gene ID (NCBI) 7448
RRIDAB_2881772
Conjugate Unconjugated
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Vitronectin is a glycoprotein present in blood and in the extracellular matrix (PMID: 10399314). Vitronectin is a multifunctional glycoprotein that mediates cell-to-substrate adhesion, inhibits the cytolytic action of the terminal complement cascade in vitro and binds to several serine protease inhibitors of the serpin family (PMID: 1372588).