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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Vertnin Polyclonal antibody, PBS Only

Vertnin Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 24824-1-PBS

Synonyms

Validation Data Gallery

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  • Western Blot (WB) analysis of SGC-7901 cells using Vertnin Polyclonal antibody (24824-1-AP)

    WB analysis of SGC-7901 using 24824-1-AP (same clone as 24824-1-PBS)

    SGC-7901 cells were subjected to SDS PAGE followed by western blot with 24824-1-AP (Vertnin antibody at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24824-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Vertnin Polyclonal antibody (24824-1-AP)

    IHC staining of human placenta using 24824-1-AP (same clone as 24824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24824-1-AP (Vertnin antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24824-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using Vertnin Polyclonal antibody (24824-1-AP)

    IHC staining of human placenta using 24824-1-AP (same clone as 24824-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 24824-1-AP (Vertnin antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 24824-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using Vertnin Polyclonal antibody (24824-1-AP)

    IF Staining of HepG2 using 24824-1-AP (same clone as 24824-1-PBS)

    Immunofluorescent analysis of HepG2 cells using 24824-1-AP (Vertnin antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG. This data was developed using the same antibody clone with 24824-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24824-1-PBS targets Vertnin in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vertnin fusion protein Ag20678 相同性解析による交差性が予測される生物種
Full Name chromosome 14 open reading frame 115
Calculated molecular weight 702 aa, 78 kDa
Observed molecular weight 80 kDa
GenBank accession numberBC053325
Gene symbol Vertnin
Gene ID (NCBI) 55237
RRIDAB_2879745
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

Vertnin, also named as VRTN and C14orf115, belongs to the vertnin family. It is an essential factor for development of the embryo in a wide range of organisms.