- Featured Product
- KD/KO Validated
CoraLite®488-conjugated Vimentin Monoclonal antibody
Vimentin Monoclonal Antibody for IF/ICC, IF-P
Host / Isotype
Mouse / IgG1
Reactivity
human, mouse, rat
Applications
IHC, IF/ICC, IF-P
Conjugate
CoraLite®488 Fluorescent Dye
CloneNo.
3H9D1
Cat no : CL488-60330
Synonyms
Validation Data Gallery
Tested Applications
Positive IF-P detected in | human tonsillitis tissue |
Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
CL488-60330 targets Vimentin in IHC, IF/ICC, IF-P applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | Vimentin fusion protein Ag0489 相同性解析による交差性が予測される生物種 |
Full Name | vimentin |
Calculated molecular weight | 466 aa, 54 kDa |
GenBank accession number | BC000163 |
Gene symbol | VIM |
Gene ID (NCBI) | 7431 |
RRID | AB_2883145 |
Conjugate | CoraLite®488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 491 nm / 516 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vimentin, also named as VIM, belongs to the intermediate filament family. Vimentin is class-III intermediate filaments found in various non-epithelial cells, especially mesenchymal cells. Vimentin is important for stabilizing the architecture of the cytoplasm. Monocyte-derived macrophages secrete vimentin into the extracellular space in vitro. Secretion of vimentin was enhanced by the proinflammatory cytokine tumor necrosis factor-alpha (TNFA; 191160) and inhibited by the antiinflammatory cytokine IL10 (124092), suggesting that vimentin is involved in the immune response. Vimentin has specialized functions that contribute to specific dynamic cellular processes. As a phosphoprotein, 55-60 kDa of vimentin proteins can be observed due to the different phosphorylation level. The antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.
Protocols
Product Specific Protocols | |
---|---|
IF protocol for CL488 Vimentin antibody CL488-60330 | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Signal Transduct Target Ther Circulating tumor cells shielded with extracellular vesicle-derived CD45 evade T cell attack to enable metastasis | ||
J Mol Cell Biol Cholesterol metabolic enzyme Ggpps regulates epicardium development and ventricular wall architecture integrity in mice. |