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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite®594-conjugated Vinculin Polyclonal antibody

Vinculin Polyclonal Antibody for IF

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

Cat no : CL594-26520

Synonyms

CMD1W, Metavinculin, MVCL, VCL, vinculin

Validation Data Gallery

Applications:
  • IF Staining of A549 using CL594-26520

    IF Staining of A549 using CL594-26520

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 Vinculin antibody (CL594-26520) at dilution of 1:400, CoraLite®594 Beta Actin antibody (CL594-66009, Clone: 2D4H5, red).

  • IF Staining of HUVEC using CL594-26520

    IF Staining of HUVEC using CL594-26520

    Immunofluorescent analysis of (-20°C Methanol) fixed HUVEC cells using CoraLite®594 Vinculin antibody (CL594-26520) at dilution of 1:200.

Tested Applications

Positive IF detected inA549 cells, HUVEC cells
For other applications, we recommend the unconjugated version of this antibody, 26520-1-AP

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-26520 is Vinculin Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Vinculin fusion protein Ag24942 相同性解析による交差性が予測される生物種
Full Name vinculin
Calculated molecular weight 1133 aa, 124 kDa
Observed molecular weight 117 kDa
GenBank accession numberBC039174
Gene symbol VCL
Gene ID (NCBI) 7414
RRIDAB_2919877
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

Background Information

Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.

Protocols

Product Specific Protocols
IF protocol for CL594 Vinculin antibody CL594-26520Download protocol
Standard Protocols
Click here to view our Standard Protocols