CoraLite®594-conjugated Vinculin Polyclonal antibody
Vinculin Polyclonal Antibody for IF/ICC
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse
Applications
IF/ICC
Conjugate
CoraLite®594 Fluorescent Dye
Cat no : CL594-26520
Synonyms
Validation Data Gallery
Tested Applications
Positive IF/ICC detected in | A549 cells, HUVEC cells |
Recommended dilution
Application | Dilution |
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Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL594-26520 targets Vinculin in IF/ICC applications and shows reactivity with human, mouse samples.
Tested Reactivity | human, mouse |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | Vinculin fusion protein Ag24942 相同性解析による交差性が予測される生物種 |
Full Name | vinculin |
Calculated molecular weight | 1133 aa, 124 kDa |
Observed molecular weight | 117 kDa |
GenBank accession number | BC039174 |
Gene symbol | Vinculin |
Gene ID (NCBI) | 7414 |
RRID | AB_2919877 |
Conjugate | CoraLite®594 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 588 nm / 604 nm |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vinculin belongs to the vinculin/alpha-catenin family. It is an actin filament (F-actin)-binding protein which involved in cell-matrix adhesion and cell-cell adhesion. Vinculin regulates cell-surface E-cadherin expression and potentiates mechanosensing by the E-cadherin complex. It may also play important roles in cell morphology and locomotion. Vinculin is a 117-kDa, 1,066-amino-acid protein which is ubiquitously expressed. Its splice variant, metavinculin (124 kDa), is muscle-specific.
Protocols
Product Specific Protocols | |
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IF protocol for CL594 Vinculin antibody CL594-26520 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |