Vitamin D binding protein Monoclonal antibody
Vitamin D binding protein Monoclonal Antibody for WB, IHC, FC (Intra), ELISA
Host / Isotype
Mouse / IgG1
Reactivity
human and More (1)
Applications
WB, IHC, FC (Intra), CoIP, ChIP, ELISA, IF
Conjugate
Unconjugated
CloneNo.
1E4D10
Cat no : 66175-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | human testis tissue |
| Positive IHC detected in | human liver tissue, human colon cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | U-937 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
| CoIP | See 1 publications below |
| ChIP | See 1 publications below |
Product Information
66175-1-Ig targets Vitamin D binding protein in WB, IHC, FC (Intra), CoIP, ChIP, ELISA, IF applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Vitamin D binding protein fusion protein Ag9803 相同性解析による交差性が予測される生物種 |
| Full Name | group-specific component (vitamin D binding protein) |
| Calculated molecular weight | 474 aa, 53 kDa |
| Observed molecular weight | 52-58 kDa |
| GenBank accession number | BC057228 |
| Gene symbol | Vitamin D binding protein |
| Gene ID (NCBI) | 2638 |
| RRID | AB_2881570 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Vitamin D binding protein antibody 66175-1-Ig | Download protocol |
| IHC protocol for Vitamin D binding protein antibody 66175-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
