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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated Vitamin D binding protein Monoclonal antibody

Vitamin D binding protein Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1E4D10

Cat no : CL488-66175

Synonyms

GC, VDBP,GC, Gc globulin, DBP-maf, DBP/GC

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U-937 cells using CoraLite® Plus 488-conjugated Vitamin D binding pr (CL488-66175)

    IF Staining of U-937 using CL488-66175

    Immunofluorescent analysis of (4% PFA) fixed U-937 cells using CoraLite® Plus 488 Vitamin D binding protein antibody (CL488-66175, Clone: 1E4D10 ) at dilution of 1:100.

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Tested Applications

Positive IF/ICC detected inU-937 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66175 targets Vitamin D binding protein in IF/ICC applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Vitamin D binding protein fusion protein Ag9803 相同性解析による交差性が予測される生物種
Full Name group-specific component (vitamin D binding protein)
Calculated molecular weight 474 aa, 53 kDa
Observed molecular weight 52-58 kDa
GenBank accession numberBC057228
Gene symbol Vitamin D binding protein
Gene ID (NCBI) 2638
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Vitamin D binding protein is a sparsely glycosylated serum protein responsible for highly specific binding and tissue-specific delivery of vitamin D and its metabolites. In addition, it is also an actin scavenger, and is the precursor to the immunomodulatory protein, Gc-MAF. Vitamin D binding protein has been proposed to have significant roles in C5a chemotaxis, osteoclast development and possibly in macrophage activation/recruitment.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 Vitamin D binding protein antibody CL488-66175Download protocol
Standard Protocols
Click here to view our Standard Protocols