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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WAPL Recombinant antibody (84877-5-RR)

    WB analysis using 84877-5-RR (same clone as 84877-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84877-5-RR (WAPL antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using WAPL Recombinant antibody (84877-5-RR)

    IHC staining of mouse skeletal muscle using 84877-5-RR (same clone as 84877-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84877-5-RR (WAPL antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse skeletal muscle tissue using WAPL Recombinant antibody (84877-5-RR)

    IHC staining of mouse skeletal muscle using 84877-5-RR (same clone as 84877-5-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84877-5-RR (WAPL antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using WAPL Recombinant antibody (84877-5-RR)

    IF Staining of HepG2 using 84877-5-RR (same clone as 84877-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using WAPL antibody (84877-5-RR, Clone: 242443C1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using WAPL Recombinant antibody (84877-5-RR)

    FC experiment of HeLa using 84877-5-RR (same clone as 84877-5-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug WAPL Recombinant antibody (84877-5-RR, Clone:242443C1) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84877-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84877-5-RR

    Affinity and Kinetic Characterization of 84877-5-RR

    Biolayer interferometry (BLl) kinetic assays of 84877-5-RR against Human WAPL were performed. The affinity constant is 0.23 nM.

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WAPL Recombinant antibody, PBS Only

WAPL Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84877-5-PBS
Clone No.242443C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Synonyms

WAPL/FOE, FOE, 242443C1, WAPL cohesin release factor, WAPAL

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84877-5-PBS targets WAPL in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen WAPL fusion protein Ag9585 相同性解析による交差性が予測される生物種
Full Name wings apart-like homolog (Drosophila)
Calculated molecular weight 142 kDa
Observed molecular weight 160-180 kDa
GenBank accession numberBC017393
Gene Symbol WAPL
Gene ID (NCBI) 23063
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ7Z5K2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

The wings apart-like (WAPL, or WAPAL) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. Human WAPL is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. WAPAL has a predicted molecular weight of 140 kDa, however, as a result of its extensive phosphorylation it separates on a SDS gel as a band of approximately 160-180 kDa.