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WAPL Recombinant antibody

WAPL Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84877-5-RR
Clone No.242443C1

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

WAPL/FOE, FOE, 242443C1, WAPL cohesin release factor, WAPAL

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -


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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター(国内)に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。


保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換/補填対応を承ります。詳細はこちらをご覧ください。


Tested Applications

Positive WB detected inU2OS cells, MCF-7 cells, K-562 cells, HepG2 cells, HeLa cells, HEK-293 cells
Positive IHC detected inmouse skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

84877-5-RR targets WAPL in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen WAPL fusion protein Ag9585 相同性解析による交差性が予測される生物種
Full Name wings apart-like homolog (Drosophila)
Calculated molecular weight 142 kDa
Observed molecular weight 160-180 kDa
GenBank accession numberBC017393
Gene Symbol WAPL
Gene ID (NCBI) 23063
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ7Z5K2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The wings apart-like (WAPL, or WAPAL) protein was originally identified as a gene product that potentially regulates heterochromatin organization in Drosophila melanogaster. Human WAPL is a cohesin-binding protein that promotes sister-chromatid resolution in mitotic prophase. Overexpression of WAPL disturbs mitosis and cytokinesis, and contributes to tumor progression by induction of chromosomal instability. WAPAL has a predicted molecular weight of 140 kDa, however, as a result of its extensive phosphorylation it separates on a SDS gel as a band of approximately 160-180 kDa.

Protocols

Product Specific Protocols
WB protocol for WAPL antibody 84877-5-RRDownload protocol
IHC protocol for WAPL antibody 84877-5-RRDownload protocol
IF protocol for WAPL antibody 84877-5-RRDownload protocol
FC protocol for WAPL antibody 84877-5-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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