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Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WDFY4 Polyclonal antibody, PBS Only

WDFY4 Polyclonal Antibody for IHC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IHC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 17558-1-PBS

Synonyms

Validation Data Gallery

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  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using WDFY4 Polyclonal antibody (17558-1-AP)

    IHC staining of human tonsillitis using 17558-1-AP (same clone as 17558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using WDFY4 Polyclonal antibody (17558-1-AP)

    IHC staining of human tonsillitis using 17558-1-AP (same clone as 17558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using WDFY4 Polyclonal antibody (17558-1-AP)

    IHC staining of mouse brain using 17558-1-AP (same clone as 17558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using WDFY4 Polyclonal antibody (17558-1-AP)

    IHC staining of mouse brain using 17558-1-AP (same clone as 17558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using WDFY4 Polyclonal antibody (17558-1-AP)

    IHC staining of human prostate cancer using 17558-1-AP (same clone as 17558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17558-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using WDFY4 Polyclonal antibody (17558-1-AP)

    IHC staining of human prostate cancer using 17558-1-AP (same clone as 17558-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 17558-1-AP (WDFY4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17558-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

17558-1-PBS targets WDFY4 in IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDFY4 fusion protein Ag11720 相同性解析による交差性が予測される生物種
Full Name WDFY family member 4
Calculated molecular weight 354 kDa
Observed molecular weight ~70 kDa
GenBank accession numberBC032420
Gene symbol WDFY4
Gene ID (NCBI) 57705
RRIDAB_2288447
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

WDFY4, also named as C10orf64 and KIAA1607, is a huge protein with unknown functions. WDFY4 is expressed predominantly in immune tissues such as lymph nodes, the spleen, the thymus and the tonsils. According to recent genome-wide association studies (GWAS), WDFY4 was introduced as novel predisposing genes for SLE (Systemic Lupus Erythematosus) which is a heterogeneous complex relapsing-remitting autoimmune disease. This antibody detects the C-terminal of WDFY4. The gene WDFY4 has some isoforms, 354 kDa, 73 kDa, 115 kDa, 353 kDa, 325 kDa( from UNIPROT) and 1992aa, 222 kDa; 3224aa, 358kDa; 624aa, 69 kDa; 1270aa, 143 kDa; 1887aa, 211 kDa (From NCBI).