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Validation Data Gallery

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  • Western Blot (WB) analysis of mouse skeletal muscle tissue using WDR45/WIPI4 Polyclonal antibody (19194-1-AP)

    WB analysis of mouse skeletal muscle using 19194-1-AP (same clone as 19194-1-PBS)

    mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using WDR45/WIPI4 Polyclonal antibody (19194-1-AP)

    WB analysis using 19194-1-AP (same clone as 19194-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 19194-1-AP (WDR45 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using WDR45/WIPI4 Polyclonal antibody (19194-1-AP)

    IHC staining of human liver using 19194-1-AP (same clone as 19194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using WDR45/WIPI4 Polyclonal antibody (19194-1-AP)

    IHC staining of human liver using 19194-1-AP (same clone as 19194-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 19194-1-AP (WDR45 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WDR45/WIPI4 Polyclonal antibody (19194-1-AP)

    IF Staining of HeLa using 19194-1-AP (same clone as 19194-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using WDR45/WIPI4 antibody (19194-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using WDR45/WIPI4 Polyclonal antibody (19194-1-AP)

    IF Staining of HeLa using 19194-1-AP (same clone as 19194-1-PBS)

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HeLa cells using 19194-1-AP (WDR45 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 19194-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

WDR45/WIPI4 Polyclonal antibody, PBS Only

WDR45/WIPI4 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 19194-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

WDR45, WDRX1, WD repeat-containing protein 45, WD repeat domain phosphoinositide-interacting protein 4, WD repeat domain 45

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

19194-1-PBS targets WDR45/WIPI4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WDR45/WIPI4 fusion protein Ag6741 相同性解析による交差性が予測される生物種
Full Name WD repeat domain 45
Calculated molecular weight 40 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC000464
Gene Symbol WDR45
Gene ID (NCBI) 11152
RRIDAB_2215404
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9Y484
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

WD repeat domain phosphoinositide-interacting protein 4 (WDR45) is also named as WDRX1, WDRXI4 and WIPI4, and belongs to the WD repeat PROPPIN family. WDR45 is highly conserved in mammals, and the amino acid sequences of the human, mouse, pig, bovine and horse protein have more than 97% identity (PMID:20505359). WDR45 is component of the autophagy machinery that controls the major intracellular degradation process (PMID:23435086, PMID:28561066). Together with WIPI1, WIPI2 and WIPI3/WDR45B, it forms the WIPI (WD repeat domain, phosphoinositide interacting) protein family. And this is why WDR45 is also known as WIPI4 (PMID:11814058). The best characterized molecular function of WDR45 is promoting lipid transfer together with ATG2 proteins between adjacent membranes. This function at the phagophore-ER membrane contact sites is important for autophagy (PMID:30185561, PMID:31721365). WDR45 is activated by the STK11/AMPK signaling pathway upon starvation. WDR45 is involved in autophagosome assembly downstream of WIPI2, regulating the size of forming autophagosomes (PMID:28561066).