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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated WIPI2 Polyclonal antibody

WIPI2 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-28820

Synonyms

IDDSSA, CGI-50, CGI50, CGI 50, Atg21

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using CoraLite® Plus 488-conjugated WIPI2 Polyclonal ant (CL488-28820)

    IF Staining of HeLa using CL488-28820

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 WIPI2 antibody (CL488-28820) at dilution of 1:200.

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Tested Applications

Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-28820 targets WIPI2 in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WIPI2 fusion protein Ag30505 相同性解析による交差性が予測される生物種
Full Name WD repeat domain, phosphoinositide interacting 2
Calculated molecular weight 49 kDa
Observed molecular weight 49 kDa
GenBank accession numberBC004116
Gene symbol WIPI2
Gene ID (NCBI) 26100
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WIPI2, the mammalian homologue of the yeast ATG18 gene, plays a key role in autophagy. It binds the omegasome, a phosphatidylinositol 3-phosphate (PI3P) rich domain of the endoplasmic reticulum from which the mature autophagosome develops. Western blotting detected a 49 kDa WIPI2 band.(PMID: 30968111, PMID: 30403914)

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 WIPI2 antibody CL488-28820Download protocol
Standard Protocols
Click here to view our Standard Protocols