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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WNT8A Polyclonal antibody

WNT8A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 30518-1-AP

Synonyms

WNT8D, Protein Wnt-8d, Protein Wnt-8a, Protein Wnt 8d, Protein Wnt 8a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WNT8A Polyclonal antibody (30518-1-AP)

    WB analysis using 30518-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 30518-1-AP (WNT8A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using WNT8A Polyclonal antibody (30518-1-AP)

    IHC staining of human colon cancer using 30518-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30518-1-AP (WNT8A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using WNT8A Polyclonal antibody (30518-1-AP)

    IF Staining of HCT 116 using 30518-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using WNT8A antibody (30518-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

Positive WB detected inCaco-2 cells, HT-29 cells, SW480 cells, rat heart tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

30518-1-AP targets WNT8A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT8A fusion protein Ag29510 相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 8A
Calculated molecular weight 39 kDa
Observed molecular weight 39-45 kDa
GenBank accession numberNM_001300938
Gene symbol WNT8A
Gene ID (NCBI) 7478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WNT8A,also named as WNT8D, belongs to the Wnt family of secreted signaling proteins. It is a ligand for members of the frizzled family of seven transmembrane receptors. These have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT8A may play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.

Protocols

Product Specific Protocols
WB protocol for WNT8A antibody 30518-1-APDownload protocol
IHC protocol for WNT8A antibody 30518-1-APDownload protocol
IF protocol for WNT8A antibody 30518-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols