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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using CoraLite® Plus 488-conjugated WNT8A Polyclonal ant (CL488-30518)

    IF Staining of HCT 116 using CL488-30518

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using CoraLite® Plus 488 WNT8A antibody (CL488-30518) at dilution of 1:200, CL594-Phalloidin (red).

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using CoraLite® Plus 488-conjugated WNT8A Polyclonal ant (CL488-30518)

    IF Staining of HCT 116 using CL488-30518

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using CoraLite® Plus 488 WNT8A antibody (CL488-30518) at dilution of 1:200, CL594-Phalloidin (red).

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CoraLite® Plus 488-conjugated WNT8A Polyclonal antibody

WNT8A Polyclonal Antibody for IF/ICC
Cat No. CL488-30518

Host / Isotype

Rabbit / IgG

Reactivity

Human, rat

Applications

IF/ICC

Synonyms

Protein Wnt 8a, Protein Wnt 8d, WNT8A, WNT8D

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inHCT 116 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-30518 targets WNT8A in IF/ICC applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT8A fusion protein Ag29510 相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 8A
Calculated molecular weight 39 kDa
Observed molecular weight 39-45 kDa
GenBank accession numberNM_001300938
Gene Symbol WNT8A
Gene ID (NCBI) 7478
RRIDAB_3672848
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H1J5
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

WNT8A,also named as WNT8D, belongs to the Wnt family of secreted signaling proteins. It is a ligand for members of the frizzled family of seven transmembrane receptors. These have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT8A may play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 WNT8A antibody CL488-30518Download protocol
Standard Protocols
Click here to view our Standard Protocols