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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

WNT8A Polyclonal antibody, PBS Only

WNT8A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugate

Unconjugated

Cat no : 30518-1-PBS

Synonyms

WNT8D, Protein Wnt-8d, Protein Wnt-8a, Protein Wnt 8d, Protein Wnt 8a

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using WNT8A Polyclonal antibody (30518-1-AP)

    WB analysis using 30518-1-AP (same clone as 30518-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 30518-1-AP (WNT8A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 30518-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using WNT8A Polyclonal antibody (30518-1-AP)

    IHC staining of human colon cancer using 30518-1-AP (same clone as 30518-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 30518-1-AP (WNT8A antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0). This data was developed using the same antibody clone with 30518-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HCT 116 cells using WNT8A Polyclonal antibody (30518-1-AP)

    IF Staining of HCT 116 using 30518-1-AP (same clone as 30518-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HCT 116 cells using WNT8A antibody (30518-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 30518-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

30518-1-PBS targets WNT8A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WNT8A fusion protein Ag29510 相同性解析による交差性が予測される生物種
Full Name wingless-type MMTV integration site family, member 8A
Calculated molecular weight 39 kDa
Observed molecular weight 39-45 kDa
GenBank accession numberNM_001300938
Gene symbol WNT8A
Gene ID (NCBI) 7478
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

WNT8A,also named as WNT8D, belongs to the Wnt family of secreted signaling proteins. It is a ligand for members of the frizzled family of seven transmembrane receptors. These have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. WNT8A may play an important role in the development and differentiation of certain forebrain structures, notably the hippocampus.