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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using CoraLite® Plus 488-conjugated WWTR1 Polyclonal ant (CL488-23306)

    IF Staining of U-251 using CL488-23306

    Immunofluorescent analysis of (4% PFA) fixed U-251 cells using CoraLite® Plus 488 WWTR1 antibody (CL488-23306) at dilution of 1:200, CL594-phalloidin (red).

  • Flow cytometry (FC) experiment of HeLa cells using CoraLite® Plus 488-conjugated WWTR1 Polyclonal ant (CL488-23306)

    FC experiment of HeLa using CL488-23306

    1x10^6 HeLa cells were intracellularly stained with 0.8 ug CoraLite® Plus 488 Anti-Human WWTR1 (CL488-23306)(red), or 0.8 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated WWTR1/TAZ Polyclonal antibody

WWTR1/TAZ Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL488-23306

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

IF/ICC, FC (Intra)

Synonyms

TAZ, WWTR1, WW domain-containing transcription regulator protein 1, Transcriptional coactivator with PDZ-binding motif

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inU-251 cells
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-23306 targets WWTR1/TAZ in IF/ICC, FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen WWTR1/TAZ fusion protein Ag19659 相同性解析による交差性が予測される生物種
Full Name WW domain containing transcription regulator 1
Calculated molecular weight 44 kDa
Observed molecular weight 50 kDa
GenBank accession numberBC014052
Gene Symbol WWTR1
Gene ID (NCBI) 25937
RRIDAB_3672754
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9GZV5
Storage Buffer PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TAZ, also referred as WWTR1, is a transcriptional co-activator with a PDZ-binding motif that is regulated by its interaction with 14-3-3 protein. TAZ is expressed in many primary tumors, such as breast cancer, thyroid carcinoma, colorectal cancer, and glioma. TAZ has been reported to be one of the nuclear effectors of Hippo-related pathways that regulate organ size control, cancer stem cell (CSC) properties, and epithelial-mesenchymal transition (EMT). TAZ has been defined for its role in the nucleus, where it functions directly as a transcriptional regulator by interacting with several nuclear factors as Runx2/Cbfa1, NHERF-2, TEF-1, TBX5, PAX3, PAX8 and TTF-1.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 WWTR1/TAZ antibody CL488-23306Download protocol
FC protocol for CL Plus 488 WWTR1/TAZ antibody CL488-23306Download protocol
Standard Protocols
Click here to view our Standard Protocols