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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

XPA Polyclonal antibody, PBS Only

XPA Polyclonal Antibody for WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, FC (Intra), IP, Indirect ELISA

Conjugate

Unconjugated

Cat no : 16462-1-PBS

Synonyms

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of L02 cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of L02 using 16462-1-AP (same clone as 16462-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of L02 using 16462-1-AP (same clone as 16462-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of HeLa using 16462-1-AP (same clone as 16462-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of HeLa using 16462-1-AP (same clone as 16462-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of MCF-7 using 16462-1-AP (same clone as 16462-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of MCF-7 using 16462-1-AP (same clone as 16462-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of HEK-293 using 16462-1-AP (same clone as 16462-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using XPA Polyclonal antibody (16462-1-AP)

    WB analysis of HeLa using 16462-1-AP (same clone as 16462-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 16462-1-AP (XPA Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of L02 cells using XPA Polyclonal antibody (16462-1-AP)

    IP experiment of L02 using 16462-1-AP (same clone as 16462-1-PBS)

    IP result of anti-XPA (IP:16462-1-AP, 4ug; Detection:16462-1-AP 1:300) with L02 cells lysate 3000 ug. This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using XPA Polyclonal antibody (16462-1-AP)

    IF Staining of HepG2 using 16462-1-AP (same clone as 16462-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using XPA antibody (16462-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HepG2 cells using XPA Polyclonal antibody (16462-1-AP)

    FC experiment of HepG2 using 16462-1-AP (same clone as 16462-1-PBS)

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human XPA (16462-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 16462-1-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

16462-1-PBS targets XPA in WB, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen XPA fusion protein Ag9536 相同性解析による交差性が予測される生物種
Full Name xeroderma pigmentosum, complementation group A
Calculated molecular weight374aa,42 kDa; 273aa,31 kDa
Observed molecular weight 40 kDa
GenBank accession numberBC014965
Gene symbol XPA
Gene ID (NCBI) 7507
RRIDAB_2878261
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

XPA, also named as DNA repair protein complementing XP-A cells, is a 272 amino acid protein, which belongs to the XPA family. XPA is expressed in various cell lines and in skin fibroblasts. XPA is involved in DNA excision repair. Initiates repair by binding to damaged sites with various affinities, depending on the photoproduct and the transcriptional state of the region. XPA is required for UV-induced CHEK1 phosphorylation and the recruitment of CEP164 to cyclobutane pyrimidine dimmers (CPD), sites of DNA damage after UV irradiation. The calculated molecular weight of XPA is 31 kDa, but modified XPA protein is about 40 kDa (PMID: 17848622).