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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using XRCC4 Monoclonal antibody (66621-1-Ig)

    WB analysis of HEK-293 using 66621-1-Ig

    WB result of XRCC4 antibody (66621-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XRCC4 transfected HEK-293 cells.

  • Western Blot (WB) analysis of various lysates using XRCC4 Monoclonal antibody (66621-1-Ig)

    WB analysis using 66621-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of COLO 320 cells using XRCC4 Monoclonal antibody (66621-1-Ig)

    WB analysis of COLO 320 using 66621-1-Ig

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using XRCC4 Monoclonal antibody (66621-1-Ig)

    WB analysis of MCF-7 using 66621-1-Ig

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using XRCC4 Monoclonal antibody (66621-1-Ig)

    IHC staining of human colon cancer using 66621-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using XRCC4 Monoclonal antibody (66621-1-Ig)

    IHC staining of human colon cancer using 66621-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using XRCC4 Monoclonal antibody (66621-1-Ig)

    IHC staining of human breast cancer using 66621-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using XRCC4 Monoclonal antibody (66621-1-Ig)

    IHC staining of human breast cancer using 66621-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  • Featured Product
  • KD/KO Validated

XRCC4 Monoclonal antibody

XRCC4 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66621-1-Ig
Clone No.3E5D2
Publications(1)

Host / Isotype

Mouse / IgG2b

Reactivity

Human and More (1)

Applications

WB, IHC, ELISA

Synonyms

DNA repair protein XRCC4, XRCC4

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, COLO 320 cells, MCF-7 cells, A431 cells, HepG2 cells
Positive IHC detected inhuman colon cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

66621-1-Ig targets XRCC4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen XRCC4 fusion protein Ag8807 相同性解析による交差性が予測される生物種
Full Name X-ray repair complementing defective repair in Chinese hamster cells 4
Calculated molecular weight 310 aa, 35 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC005259
Gene Symbol XRCC4
Gene ID (NCBI) 7518
RRIDAB_2881981
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13426
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for XRCC4 antibody 66621-1-IgDownload protocol
IHC protocol for XRCC4 antibody 66621-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Immunity

Cytoplasmic DNA sensing by KU complex in aged CD4+ T cell potentiates T cell activation and aging-related autoimmune inflammation.

Authors - Yan Wang
  • KD Validated
View Article