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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using XRCC4 Monoclonal antibody (66621-1-Ig)

    WB analysis using 66621-1-Ig (same clone as 66621-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using XRCC4 Monoclonal antibody (66621-1-Ig)

    WB analysis of HEK-293 using 66621-1-Ig (same clone as 66621-1-PBS)

    WB result of XRCC4 antibody (66621-1-Ig; 1:5000; incubated at room temperature for 1.5 hours) with sh-Control and sh-XRCC4 transfected HEK-293 cells. This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of COLO 320 cells using XRCC4 Monoclonal antibody (66621-1-Ig)

    WB analysis of COLO 320 using 66621-1-Ig (same clone as 66621-1-PBS)

    COLO 320 cells were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using XRCC4 Monoclonal antibody (66621-1-Ig)

    WB analysis of MCF-7 using 66621-1-Ig (same clone as 66621-1-PBS)

    MCF-7 cells were subjected to SDS PAGE followed by western blot with 66621-1-Ig (XRCC4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using XRCC4 Monoclonal antibody (66621-1-Ig)

    IHC staining of human colon cancer using 66621-1-Ig (same clone as 66621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using XRCC4 Monoclonal antibody (66621-1-Ig)

    IHC staining of human colon cancer using 66621-1-Ig (same clone as 66621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using XRCC4 Monoclonal antibody (66621-1-Ig)

    IHC staining of human breast cancer using 66621-1-Ig (same clone as 66621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using XRCC4 Monoclonal antibody (66621-1-Ig)

    IHC staining of human breast cancer using 66621-1-Ig (same clone as 66621-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66621-1-Ig (XRCC4 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66621-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

XRCC4 Monoclonal antibody, PBS Only

XRCC4 Monoclonal Antibody for WB, IHC, ELISA
Cat No. 66621-1-PBS
Clone No.3E5D2

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

WB, IHC, ELISA

Synonyms

DNA repair protein XRCC4, XRCC4

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

66621-1-PBS targets XRCC4 in WB, IHC, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen XRCC4 fusion protein Ag8807 相同性解析による交差性が予測される生物種
Full Name X-ray repair complementing defective repair in Chinese hamster cells 4
Calculated molecular weight 310 aa, 35 kDa
Observed molecular weight 55 kDa
GenBank accession numberBC005259
Gene Symbol XRCC4
Gene ID (NCBI) 7518
RRIDAB_2881981
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13426
Storage Buffer PBS only
Storage ConditionsStore at -80°C.