CoraLite® Plus 488-conjugated XRN2 Monoclonal antibody
XRN2 Monoclonal Antibody for FC (Intra)
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse, rat
Applications
FC (Intra)
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2C3E3
Cat no : CL488-66852
Synonyms
Validation Data Gallery
Tested Applications
Positive FC (Intra) detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
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Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
CL488-66852 targets XRN2 in FC (Intra) applications and shows reactivity with Human, mouse, rat samples.
Tested Reactivity | Human, mouse, rat |
Host / Isotype | Mouse / IgG1 |
Class | Monoclonal |
Type | Antibody |
Immunogen | XRN2 fusion protein Ag27927 相同性解析による交差性が予測される生物種 |
Full Name | 5'-3' exoribonuclease 2 |
Calculated molecular weight | 104 kDa |
Observed molecular weight | 109 kDa |
GenBank accession number | BC006417 |
Gene symbol | XRN2 |
Gene ID (NCBI) | 22803 |
Conjugate | CoraLite® Plus 488 Fluorescent Dye |
Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
Form | Liquid |
Purification Method | Protein G purification |
Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
XRN2 is one exonuclease that degrades the Pol II associated product of poly(A) site cleavage, which is crucial for Pol II termination. During transcription termination, XRN2 cleavages at the polyadenylation site liberates a 5' fragment which is subsequently processed to form the mature mRNA and a 3' fragment which remains attached to the elongating polymerase. The processive degradation of this 3' fragment by this protein may promote termination of transcription.
Protocols
Product Specific Protocols | |
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FC protocol for CL Plus 488 XRN2 antibody CL488-66852 | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |