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Host
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Species Reactivity
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Species Reactivity
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Conjugate
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Conjugate
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Conjugate
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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

YAF2 Recombinant antibody, PBS Only

YAF2 Recombinant Antibody for WB, FC (Intra), ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

240834G11

Cat no : 83798-6-PBS

Synonyms

YY1-associated factor 2, YY1 associated factor 2, 240834G11

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YAF2 Recombinant antibody (83798-6-RR)

    WB analysis using 83798-6-RR (same clone as 83798-6-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83798-6-RR (YAF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83798-6-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using YAF2 Recombinant antibody (83798-6-RR)

    FC experiment of HeLa using 83798-6-RR (same clone as 83798-6-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug YAF2 Recombinant antibody (83798-6-RR, Clone:240834G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 83798-6-PBS in a different storage buffer formulation.

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

The immunogen of 83798-6-PBS is YAF2 Fusion Protein expressed in E. coli.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen YAF2 fusion protein Ag5023 相同性解析による交差性が予測される生物種
Full Name YY1 associated factor 2
Calculated molecular weight 204 aa, 23 kDa
Observed molecular weight20 kDa
GenBank accession numberBC037777
Gene symbol YAF2
Gene ID (NCBI) 10138
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS Only
Storage ConditionsStore at -80°C.

Background Information

YY1-associated factor 2 (YAF2) is the binding molecule of Yin-Yang-1 (YY-1) protein and plays an important role in transcriptional regulation. YAF-2 mRNA expression was the highest in heart and skeletal muscle (PMID: 11953439).