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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using YAF2 Recombinant antibody (83798-6-RR)

    WB analysis using 83798-6-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83798-6-RR (YAF2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Flow cytometry (FC) experiment of HeLa cells using YAF2 Recombinant antibody (83798-6-RR)

    FC experiment of HeLa using 83798-6-RR

    1x10^6 HeLa cells were intracellularly stained with 0.25 ug YAF2 Recombinant antibody (83798-6-RR, Clone:240834G11) and APC-Conjugated Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug Isotype Control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set.

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YAF2 Recombinant antibody

YAF2 Uni-rAbTM Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 83798-6-RR
Clone No.240834G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC (Intra), ELISA

Synonyms

YY1-associated factor 2, YY1 associated factor 2, 240834G11

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, Raji cells, HeLa cells, SK-N-SH cells, SH-SY5Y cells, human heart tissue
Positive FC (Intra) detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83798-6-RR targets YAF2 in WB, FC (Intra), ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen YAF2 fusion protein Ag5023 相同性解析による交差性が予測される生物種
Full Name YY1 associated factor 2
Calculated molecular weight 204 aa, 23 kDa
Observed molecular weight20 kDa
GenBank accession numberBC037777
Gene Symbol YAF2
Gene ID (NCBI) 10138
RRIDAB_3671387
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
UNIPROT IDQ8IY57
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YY1-associated factor 2 (YAF2) is the binding molecule of Yin-Yang-1 (YY-1) protein and plays an important role in transcriptional regulation. YAF-2 mRNA expression was the highest in heart and skeletal muscle (PMID: 11953439).

Protocols

Product Specific Protocols
WB protocol for YAF2 antibody 83798-6-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols