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  • Featured Product
  • KD/KO Validated

YES1 Monoclonal antibody

YES1 Monoclonal Antibody for WB, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

CloneNo.

1A12E7

Cat no : 67196-1-Ig

Synonyms

c yes, HsT441, P61 YES, Proto oncogene c Yes, Yes, YES1

Validation Data Gallery

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  • Western Blot (WB) analysis of HepG2 cells using YES1 Monoclonal antibody (67196-1-Ig)

    WB analysis of HepG2 using 67196-1-Ig

    WB result of YES1 antibody (67196-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-YES1 transfected HepG2 cells.

  • Western Blot (WB) analysis of various lysates using YES1 Monoclonal antibody (67196-1-Ig)

    WB analysis using 67196-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67196-1-Ig (YES1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human kidney tissue using YES1 Monoclonal antibody (67196-1-Ig)

    IHC staining of human kidney using 67196-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67196-1-Ig (YES1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human kidney tissue using YES1 Monoclonal antibody (67196-1-Ig)

    IHC staining of human kidney using 67196-1-Ig

    Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 67196-1-Ig (YES1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

Positive WB detected inA549 cells, HepG2 cells, HeLa cells, LNCaP cells, A431 cells, HSC-T6 cells, PC-12 cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67196-1-Ig targets YES1 in WB, IHC, ELISA applications and shows reactivity with Human, rat samples.

Tested Reactivity Human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen YES1 fusion protein Ag5016 相同性解析による交差性が予測される生物種
Full Name v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Calculated molecular weight 61 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC048960
Gene symbol YES1
Gene ID (NCBI) 7525
RRIDAB_2918483
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.1% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

Background Information

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Protocols

Product Specific Protocols
WB protocol for YES1 antibody 67196-1-IgDownload protocol
IHC protocol for YES1 antibody 67196-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols