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  • Featured Product
  • KD/KO Validated

CoraLite® Plus 488-conjugated YES1 Monoclonal antibody

YES1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG2a

Reactivity

human, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A12E7

Cat no : CL488-67196

Synonyms

p61-Yes, P61 YES, HsT441, EC:2.7.10.2, c yes

Validation Data Gallery

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  • Flow cytometry (FC) experiment of HepG2 cells using CoraLite® Plus 488-conjugated YES1 Monoclonal anti (CL488-67196)

    FC experiment of HepG2 using CL488-67196

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human YES1 (CL488-67196, Clone:1A12E7) (red), or 0.4 ug Mouse IgG2a Isotype Control (CL488-66360-2, Clone: K11A1B2A2) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

Positive FC (Intra) detected inHepG2 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67196 targets YES1 in FC (Intra) applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen YES1 fusion protein Ag5016 相同性解析による交差性が予測される生物種
Full Name v-yes-1 Yamaguchi sarcoma viral oncogene homolog 1
Calculated molecular weight 61 kDa
Observed molecular weight 62 kDa
GenBank accession numberBC048960
Gene symbol YES1
Gene ID (NCBI) 7525
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YES1, also named as p61-Yes and YES, belongs to the protein kinase superfamily, Tyr protein kinase family and SRC subfamily. It promotes infectivity of Neisseria gonorrhoeae in epithelial cells by phosphorylating MCP/CD46. YES1 catalyzes the reaction: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 YES1 antibody CL488-67196Download protocol
Standard Protocols
Click here to view our Standard Protocols