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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated CD20 Monoclonal antibody

CD20 Monoclonal Antibody for IF/ICC, IF-P

Host / Isotype

Mouse / IgG2b

Reactivity

human

Applications

IF/ICC, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

4A7G3

Cat no : CL488-60271

Synonyms

B lymphocyte antigen CD20, B1, Bp35, CD20, LEU 16, MS4A1, MS4A1,CD20, MS4A2, S7

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated CD20 Monoclonal anti (CL488-60271)

    IF Staining of human tonsillitis using CL488-60271

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60271 (CD20 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human tonsillitis tissue using CoraLite® Plus 488-conjugated CD20 Monoclonal anti (CL488-60271)

    IF Staining of human tonsillitis using CL488-60271

    Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CL488-60271 (CD20 antibody) at dilution of 1:100.

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using CoraLite® Plus 488-conjugated CD20 Monoclonal anti (CL488-60271)

    IF Staining of human lung cancer using CL488-60271

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using CoraLite® Plus 488 CD20 antibody (CL488-60271, Clone: 4A7G3 ) at dilution of 1:100, CoraLite®555 pan-keratin antibody (CL555-26411, Gradient).

  • Immunofluorescence (IF) / fluorescent staining of Raji cells using CoraLite® Plus 488-conjugated CD20 Monoclonal anti (CL488-60271)

    IF Staining of Raji using CL488-60271

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using CoraLite® Plus 488 CD20 antibody (CL488-60271, Clone: 4A7G3 ) at dilution of 1:200.

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Tested Applications

Positive IF-P detected inhuman tonsillitis tissue, human lung cancer tissue, Raji cells
Positive IF/ICC detected inhuman tonsillitis tissue, human lung cancer tissue, Raji cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-60271 targets CD20 in IF/ICC, IF-P applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen Recombinant protein 相同性解析による交差性が予測される生物種
Full Name membrane-spanning 4-domains, subfamily A, member 1
Calculated molecular weight 297 aa, 33 kDa
GenBank accession numberBC002807
Gene symbol CD20
Gene ID (NCBI) 931
RRIDAB_2883132
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

CD20 is a 33-37 kDa transmembrane phosphoprotein. CD20 is a B-lymphocyte surface molecule that is widely expressed during B-cell ontogeny, from early pre-B-cell developmental stages until final differentiation into plasma cells. CD20 functions as calcium-permeable cation channel. It is involved in the regulation of B-cell activation and proliferation. CD20 serves as a useful target for antibody-mediated therapeutic depletion of B-cells. This antibody is conjugated with CL488(Ex/Em 488 nm/515 nm).

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 CD20 antibody CL488-60271Download protocol
Standard Protocols
Click here to view our Standard Protocols