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Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

CoraLite® Plus 488-conjugated YTHDC1 Monoclonal antibody

YTHDC1 Monoclonal Antibody for FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

FC (Intra)

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F5G11

Cat no : CL488-67911

Synonyms

YTHDC1, 1F5G11, KIAA1966, Splicing factor YT521, YT521, YT521 B, YT521-B, YTH domain-containing protein 1, YTHDC 1

Validation Data Gallery

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  • Flow cytometry (FC) experiment of A431 cells using CoraLite® Plus 488-conjugated YTHDC1 Monoclonal an (CL488-67911)

    FC experiment of A431 using CL488-67911

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Anti-Human YTHDC1 (CL488-67911, Clone:1F5G11) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Tested Applications

Positive FC (Intra) detected inA431 cells
Positive FC detected inA431 cells

Recommended dilution

ApplicationDilution
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-67911 targets YTHDC1 in FC (Intra) applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen YTHDC1 fusion protein Ag6289 相同性解析による交差性が予測される生物種
Full Name YTH domain containing 1
Calculated molecular weight 85 kDa
Observed molecular weight~100 kDa
GenBank accession numberBC053863
Gene symbol YTHDC1
Gene ID (NCBI) 91746
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YTHDC1, containing 1 YTH domain, is one of the m6A-binding proteins that can recognize and bind to the m6A methylation site and plays a specific role in gene expression. YTHDC1 is constitutively enriched in the nucleus. It regulates mRNA splicing by bridging the interactions between the trans- and cis-regulatory elements to bind targeted mRNAs. YTHDC1 mediates the export of m6A modified mRNA from the nucleus to the cytoplasm by interacting with SRSF3, which is an essential factor driving the tumorigenic process in various types of cancers including breast cancer, colon cancer, ovarian cancer, osteosarcoma, and glioblastoma (32368386).

Protocols

Product Specific Protocols
FC protocol for CL Plus 488 YTHDC1 antibody CL488-67911Download protocol
Standard Protocols
Click here to view our Standard Protocols