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  • KD/KO Validated

YTHDF3-specific Polyclonal antibody

YTHDF3-specific Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, CoIP, RIP, ELISA

Conjugate

Unconjugated

Cat no : 25537-1-AP

Synonyms

YTH domain family protein 3, YTH domain family, member 3, YTHDF3, YTHDF3-specific


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Tested Applications

Positive WB detected inA549 cells, HeLa cells, HCT 116 cells, HepG2 cells, PC-12 cells, NIH/3T3 cells
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25537-1-AP targets YTHDF3-specific in WB, IHC, IF/ICC, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, pig, chicken
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDF3-specific fusion protein Ag22374 相同性解析による交差性が予測される生物種
Full Name YTH domain family, member 3
Calculated molecular weight 585 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC052970
Gene symbol YTHDF3
Gene ID (NCBI) 253943
RRIDAB_2847817
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.

Protocols

Product Specific Protocols
WB protocol for YTHDF3-specific antibody 25537-1-APDownload protocol
IHC protocol for YTHDF3-specific antibody 25537-1-APDownload protocol
IF protocol for YTHDF3-specific antibody 25537-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

m6A facilitates hippocampus-dependent learning and memory through YTHDF1.

Authors - Hailing Shi
humanWB,CoIP

Mol Cancer

A positive feedback circuit driven by m6A-modified circular RNA facilitates colorectal cancer liver metastasis

Authors - Kaixuan Zeng
  • KD Validated
mouseWB,RIP

Cell Mol Immunol

Inhibition of METTL3 in macrophages provides protection against intestinal inflammation

Authors - Huilong Yin
humanWB,IF

Nat Cell Biol

O-GlcNAcylation determines the translational regulation and phase separation of YTHDF proteins

Authors - Yulin Chen
humanWB

Cell Res

Oncogenic AURKA-enhanced N6-methyladenosine modification increases DROSHA mRNA stability to transactivate STC1 in breast cancer stem-like cells.

Authors - Fei Peng
humanWB

Nat Commun

Circular RNA encoded MET variant promotes glioblastoma tumorigenesis

Authors - Jian Zhong
  • KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Roy (Verified Customer) (06-15-2024)

Strong signal for YTHDF3 with this antibody from HeLa extracts (1h incubation at room temperature, 1/1000 dilution)

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: HeLa
FH

Robert (Verified Customer) (01-17-2019)

1. scrambled siRNA2. YTHDF3 siRNA

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: ST cells (porcine)
YTHDF3-specific Antibody Western Blot, validation (1/1000 dilution) in ST cells (porcine) (Cat no:25537-1-AP)