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Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of PC-12 cells using CoraLite® Plus 488-conjugated YTHDF3-specific Poly (CL488-25537)

    IF Staining of PC-12 using CL488-25537

    Immunofluorescent analysis of (-20°C Ethanol) fixed PC-12 cells using CoraLite® Plus 488 YTHDF3-specific antibody (CL488-25537) at dilution of 1:200.

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CoraLite® Plus 488-conjugated YTHDF3-specific Polyclonal antibody

YTHDF3-specific Polyclonal Antibody for IF/ICC
Cat No. CL488-25537

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

IF/ICC

Synonyms

FLJ31657, YTH domain family protein 3, YTH domain family, member 3, YTHDF3

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  CoraLite® Plus 488
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive IF/ICC detected inPC-12 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-25537 targets YTHDF3-specific in IF/ICC applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen YTHDF3-specific fusion protein Ag22374 相同性解析による交差性が予測される生物種
Full Name YTH domain family, member 3
Calculated molecular weight 585 aa, 64 kDa
Observed molecular weight 64 kDa
GenBank accession numberBC052970
Gene Symbol YTHDF3
Gene ID (NCBI) 253943
RRIDAB_3672794
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission maxima wavelengths493 nm / 522 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z739
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

YTHDF3, also named as YTH domain-containing family protein 3, is a 585 amino acid protein, which contains 1 YTH domain. YTHDF3 specifically recognizes and binds N6-methyladenosine (m6A)-containing RNAs. M6A is a modification present at internal sites of mRNAs and some non-coding RNAs and plays a role in the efficiency of mRNA splicing, processing and stability. Our antibody 25537-1-AP specially reacts with YTHDF3 protein.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 YTHDF3-specific antibody CL488-25537Download protocol
Standard Protocols
Click here to view our Standard Protocols